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Identification of differentially expressed genes in lung tissues of nickel-exposed rats using suppression subtractive hybridization
Zhang J.; Zhang J.; Fan Y.; Liu L.; Li M.; Zhou Y.; Shao Z.; Shi H.; Wang Y.
2011-11-01
Source PublicationBiological Trace Element Research
ISSN01634984 15590720
Volume143Issue:2Pages:1007-1017
AbstractOccupational exposure to nickel compound, such as nickel refining, electroplating, and in conjunction with other metals, is harmful to the health, causing respiratory distress, and lung and nasal cancer. In this work, the different gene expression patterns of lung tissues from nickel-exposed rats and controls were investigated. The suppression subtractive hybridization (SSH) method was used to generate two subtracted cDNA libraries with gene transcripts differentially expressed after nickel inducing. Dot-blot hybridizations were used to confirm differential ratios of expression of obtained SSH clones. Out of 768 unique SSH clones, which were chosen randomly from the two subtraction libraries (384 of each), 319 could be verified as differentially expressed. According to blast screening and functional annotation, 28% genes in nickel-induced cDNA library were related to cell differentiation, whereas 21% in driver library were related to oxygen transport. Two novel expressed sequence tags (ESTs; NCBI Accession No. FC809414 and No. FC809411) in nickel-induced cDNA library were obtained. The genes detected in the present study are probably important genes associated with nickel-induced lung cancer. © 2010 Springer Science+Business Media, LLC.
KeywordDot-blot Expressed sequence tag Lung cancer Nickel Suppression subtractive hybridization
DOI10.1007/s12011-010-8898-4
URLView the original
Language英語
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationTongji University
Recommended Citation
GB/T 7714
Zhang J.,Zhang J.,Fan Y.,et al. Identification of differentially expressed genes in lung tissues of nickel-exposed rats using suppression subtractive hybridization[J]. Biological Trace Element Research,2011,143(2):1007-1017.
APA Zhang J.,Zhang J.,Fan Y.,Liu L.,Li M.,Zhou Y.,Shao Z.,Shi H.,&Wang Y..(2011).Identification of differentially expressed genes in lung tissues of nickel-exposed rats using suppression subtractive hybridization.Biological Trace Element Research,143(2),1007-1017.
MLA Zhang J.,et al."Identification of differentially expressed genes in lung tissues of nickel-exposed rats using suppression subtractive hybridization".Biological Trace Element Research 143.2(2011):1007-1017.
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