UM  > Institute of Chinese Medical Sciences
Comparing coagulation activity of Selaginella tamariscina before and after stir-frying process and determining the possible active constituents based on compositional variation
Zhang, Qian1; Wang, Ya-Li1; Gao, Die2; Cai, Liang1; Yang, Yi-Yao1; Hu, Yuan-Jia3; Yang, Feng-Qing1; Chen, Hua1; Xia, Zhi-Ning1
2018-01-02
Source PublicationPHARMACEUTICAL BIOLOGY
ISSN1388-0209
Volume56Issue:1Pages:67-75
Abstract

Context: Selaginella tamariscina (P. Beauv.) Spring (Selaginellaceae) (ST) has been widely used in China as a medicine for improving blood circulation. However, its processed product, S. tamariscina carbonisatus (STC), possesses opposite haemostatic activity. Objective: To comprehensively evaluate the activity of ST and STC on physiological coagulation system of rats, and seek potential active substances accounting for the activity transformation of ST during processing. Materials and methods: The 75% methanol extracts of the whole grass (fine powder) of ST and STC were prepared, respectively. Male Sprague–Dawley rats were randomly divided into five groups: control group, model group, modelþ ST group, model þ STC group and positive control group (modelþ Yunnanbaiyao). The duration of intragastric administration was 72 h at 12 h intervals. Haemorheology parameters were measured using an LB-2 A cone-plate viscometer and the existed classic methods, respectively. SC40 semi-automatic coagulation analyzer was employed to determine coagulation indices. Meanwhile, HPLC and LC-MS were applied for chemical analyses of ST and STC extracts. Results: STC shortened tail-bleeding time, increased whole blood viscosity (WBV) and plasma viscosity (PV), decreased erythrocyte sedimentation rate blood (ESR), reduced activated partial thromboplastin time (APTT) and increased the fibrinogen (FIB) content in the plasma of bleeding model rats. Although ST could shorten APTT and TT, the FIB content was significantly decreased by ST. Dihydrocaffeic acid with increased content in STC vs. ST showed haemostatic activity for promoting the platelet aggregation induced by collagen and trap-6, and reducing APTT and PT significantly with a concentration of 171.7 lM in vitro. Amentoflavone with reduced content in STC vs. ST inhibited ADP and AA-induced platelet aggregation significantly with a concentration of 40.7 lM. Discussion and conclusions: As the processed product of ST, STC showed strong haemostatic activity on bleeding rat through regulating the parameters involved in haemorheology and plasma coagulation system. Two active compounds, dihydrocaffeic acid and amentoflavone, might be partially responsible for the haemostatic and anticoagulant activity of STC and ST, respectively.

KeywordHaemorheology And Plasma Coagulation System Natural Products Activity Transformation Dihydrocaffeic Acid Amentoflavone
DOI10.1080/13880209.2017.1421673
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS IDWOS:000426939800001
PublisherTAYLOR & FRANCIS LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang, Feng-Qing
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, PR China
2.School of Pharmacy, Southwest Medical University, Luzhou, PR China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences University of Macau, Macao, PR China
Recommended Citation
GB/T 7714
Zhang, Qian,Wang, Ya-Li,Gao, Die,et al. Comparing coagulation activity of Selaginella tamariscina before and after stir-frying process and determining the possible active constituents based on compositional variation[J]. PHARMACEUTICAL BIOLOGY,2018,56(1):67-75.
APA Zhang, Qian.,Wang, Ya-Li.,Gao, Die.,Cai, Liang.,Yang, Yi-Yao.,...&Xia, Zhi-Ning.(2018).Comparing coagulation activity of Selaginella tamariscina before and after stir-frying process and determining the possible active constituents based on compositional variation.PHARMACEUTICAL BIOLOGY,56(1),67-75.
MLA Zhang, Qian,et al."Comparing coagulation activity of Selaginella tamariscina before and after stir-frying process and determining the possible active constituents based on compositional variation".PHARMACEUTICAL BIOLOGY 56.1(2018):67-75.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qian]'s Articles
[Wang, Ya-Li]'s Articles
[Gao, Die]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qian]'s Articles
[Wang, Ya-Li]'s Articles
[Gao, Die]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qian]'s Articles
[Wang, Ya-Li]'s Articles
[Gao, Die]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.