UM
A silkworm infection model to investigate Vibrio vulnificus virulence genes
Yamamoto M.1; Kashimoto T.2; Yoshimura Y.1; Tachibana N.1; Kuroda S.1; Miki Y.1; Kitabayashi S.1; Tong P.1,3; Xiao J.1,4; Tanaka K.1; Hamamoto H.5; Sekimizu K.5; Yamamoto K.1
2016-11-01
Source PublicationMolecular Medicine Reports
ISSN17913004 17912997
Volume14Issue:5Pages:4243-4247
Abstract

The halophilic marine bacterium, Vibrio vulnificus, occasionally causes fatal septicemia in immunocompromised patients. Mice are commonly used as experimental animals to investigate the virulence of V. vulnificus, however, a large number of mice are generally required for bioassays. The present study examined whether the invertebrate species, silkworms, can be used instead of mice to investigate V. vulnificus virulence. When the silkworms were inoculated with 1.2x107 colony forming units of V. vulnificus OPU1 Rf, a virulent strain of V. vulnificus, all injected silkworms died within 48 h, however, those injected with culture filtrate or diluent did not. This silkworm infection model was then used to isolate attenuated V. vulnificus mutants from 1,016 transposon inserted mutants. Consequently, a harmless mutant, SW998, was isolated. In this strain, the transposon was inserted into the rtxA gene, which is a known V. vulnificus virulence gene. In conclusion, the present study demonstrated that silkworms are useful animals for investigating the virulence of V. vulnificus.

KeywordAnimal Model Screening Silkworm Vibrio Vulnificus Virulence
DOI10.3892/mmr.2016.5782
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaOncology ; Research & Experimental Medicine
WOS SubjectOncology ; Medicine, Research & Experimental
WOS IDWOS:000387241600029
Fulltext Access
Citation statistics
Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Okayama Prefectural University
2.Kitasato University
3.Nanchang University
4.University of Tokyo
5.Universidade de Macau
Recommended Citation
GB/T 7714
Yamamoto M.,Kashimoto T.,Yoshimura Y.,et al. A silkworm infection model to investigate Vibrio vulnificus virulence genes[J]. Molecular Medicine Reports,2016,14(5):4243-4247.
APA Yamamoto M..,Kashimoto T..,Yoshimura Y..,Tachibana N..,Kuroda S..,...&Yamamoto K..(2016).A silkworm infection model to investigate Vibrio vulnificus virulence genes.Molecular Medicine Reports,14(5),4243-4247.
MLA Yamamoto M.,et al."A silkworm infection model to investigate Vibrio vulnificus virulence genes".Molecular Medicine Reports 14.5(2016):4243-4247.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yamamoto M.]'s Articles
[Kashimoto T.]'s Articles
[Yoshimura Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yamamoto M.]'s Articles
[Kashimoto T.]'s Articles
[Yoshimura Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yamamoto M.]'s Articles
[Kashimoto T.]'s Articles
[Yoshimura Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.