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Imaging granularity of leukocytes with third harmonic generation microscopy
Tsai, CK1; Chen, YS1; Wu, PC1; Hsieh, TY1; Liu, HW1; Yeh, CY2; Lin, WL1; Chia, JS2; Liu, TM1,3
2012
Source PublicationBIOMEDICAL OPTICS EXPRESS
ISSN2156-7085
Volume3Issue:9Pages:2234-2243
Abstract

Using third harmonic generation (THG) microscopy, we demonstrate that granularity differences ofleukocytes can be revealed without a label. Excited by a 1230 nm femtosecond laser, THG signals were generated at a significantly higher level in neutrophils than other mononuclear cells, whereas signals in agranular lymphocytes were one order of magnitude smaller. Interestingly, the characteristic THG features can also be observed in vivo to track the newly recruited leukocytes following lipopolysaccharide (LPS) challenge. These results suggest that label-free THG imaging may provide timely tracking of leukocyte movement without disturbing the normal cellular or physiological status. (c) 2012 Optical Society of America

DOIhttp://dx.doi.org/10.1364/BOE.3.002234
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000308861100025
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Cited Times [WOS]:25   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Natl Taiwan Univ, Inst Biomed Engn, Taipei 10617, Taiwan
2.Natl Taiwan Univ, Coll Med, Grad Inst Immunol, Taipei 10048, Taiwan
3.Natl Taiwan Univ, Mol Imaging Ctr, Taipei 10617, Taiwan
Recommended Citation
GB/T 7714
Tsai, CK,Chen, YS,Wu, PC,et al. Imaging granularity of leukocytes with third harmonic generation microscopy[J]. BIOMEDICAL OPTICS EXPRESS,2012,3(9):2234-2243.
APA Tsai, CK.,Chen, YS.,Wu, PC.,Hsieh, TY.,Liu, HW.,...&Liu, TM.(2012).Imaging granularity of leukocytes with third harmonic generation microscopy.BIOMEDICAL OPTICS EXPRESS,3(9),2234-2243.
MLA Tsai, CK,et al."Imaging granularity of leukocytes with third harmonic generation microscopy".BIOMEDICAL OPTICS EXPRESS 3.9(2012):2234-2243.
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