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A microarray platform comparison for neuroscience applications
Parrish M.L.1; Wei N.2; Duenwald S.1; Tokiwa G.Y.1; Wang Y.1; Holder D.2; Dai H.1; Zhang X.2; Wright C.1; Hodor P.2; Cavet G.1; Phillips R.L.2; Sun B.I.2; Fare T.L.1
Source PublicationJournal of Neuroscience Methods
AbstractTo address the need for high sensitivity in gene expression profiling of small neural tissue samples (~100ng total RNA), we compared a novel RT-PCR-IVT protocol using fluor-reverse pairs on inkjet oligonucleotide microarrays and an RT-IVT protocol using P labeling on nylon cDNA arrays. The comparison protocol was designed to evaluate these systems for sensitivity, specificity, reproducibility, and linearity. We developed parameters, thresholds, and testing conditions that could be used to differentiate various systems that spanned detection chemistry and instrumentation; probe number and selection criteria; and sample processing protocols. We concluded that the inkjet system had better performance in sensitivity, specificity, and reproducibility than the nylon system, and similar performance in linearity. Between these two platforms, the data indicates that the inkjet system would perform better for the transcriptional profiling of 100ng total RNA samples for neuroscience studies. © 2003 Elsevier B.V. All rights reserved.
KeywordExpression profiling Microarray Nylon membrane Platform comparison Receiver operator characteristic
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Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Rosetta Inpharmatics LLC
2.Merck Research Laboratories
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GB/T 7714
Parrish M.L.,Wei N.,Duenwald S.,et al. A microarray platform comparison for neuroscience applications[J]. Journal of Neuroscience Methods,2004,132(1):57-68.
APA Parrish M.L.,Wei N.,Duenwald S.,Tokiwa G.Y.,Wang Y.,Holder D.,Dai H.,Zhang X.,Wright C.,Hodor P.,Cavet G.,Phillips R.L.,Sun B.I.,&Fare T.L..(2004).A microarray platform comparison for neuroscience applications.Journal of Neuroscience Methods,132(1),57-68.
MLA Parrish M.L.,et al."A microarray platform comparison for neuroscience applications".Journal of Neuroscience Methods 132.1(2004):57-68.
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