UM  > Faculty of Health Sciences
Genome-wide screens for effective siRNAs through assessing the size of siRNA effects
Zhang X.D.
2008-12-01
Source PublicationBMC Research Notes
ISSN17560500
Volume1
AbstractBackground. RNA interference (RNAi) has been seen as a revolution in functional genomics and system biology. Genome-wide RNAi research relies on the development of RNAi high-throughput screening (HTS) assays. One of the most fundamental challenges in RNAi HTS is to glean biological significance from mounds of data, which relies on the development of effective analytic methods for selecting effective small interfering RNAs (siRNAs). Findings. Based on a recently proposed parameter, strictly standardized mean difference (SSMD), I propose an analytic method for genome-wide screens of effective siRNAs through assessing and testing the size of siRNA effects. Central to this method is the capability of SSMD in quantifying siRNA effects. This method has relied on normal approximation, which works only in the primary screens but not in the confirmatory screens. In this paper, I explore the non-central t-distribution property of SSMD estimates and use this property to extend the SSMD-based method so that it works effectively in either primary or confirmatory screens as well as in any HTS screens with or without replicates. The SSMD-based method maintains a balanced control of false positives and false negatives. Conclusion. The central interest in genome-wide RNAi research is the selection of effective siRNAs which relies on the development of analytic methods to measure the size of siRNA effects. The new analytic method for hit selection provided in this paper offers a good analytic tool for selecting effective siRNAs, better than current analytic methods, and thus may have broad utility in genome-wide RNAi research. © 2008 Zhang; licensee BioMed Central Ltd.
DOI10.1186/1756-0500-1-33
URLView the original
Language英語
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationMerck Research Laboratories
Recommended Citation
GB/T 7714
Zhang X.D.. Genome-wide screens for effective siRNAs through assessing the size of siRNA effects[J]. BMC Research Notes,2008,1.
APA Zhang X.D..(2008).Genome-wide screens for effective siRNAs through assessing the size of siRNA effects.BMC Research Notes,1.
MLA Zhang X.D.."Genome-wide screens for effective siRNAs through assessing the size of siRNA effects".BMC Research Notes 1(2008).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang X.D.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang X.D.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang X.D.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.