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Error rates and powers in genome-scale RNAi screens
Zhang X.D.; Marine S.D.; Ferrer M.
Source PublicationJournal of Biomolecular Screening
ISSN10870571 1552454X
AbstractFor hit selection in genome-scale RNAi research, we do not want to miss small interfering RNAs (siRNAs) with large effects; meanwhile, we do not want to include siRNAs with small or no effects in the list of selected hits. There is a strong need to control both the false-negative rate (FNR), in which the siRNAs with large effects are not selected as hits, and the restricted false-positive rate (RFPR), in which the siRNAs with no or small effects are selected as hits. An error control method based on strictly standardized mean difference (SSMD) has been proposed to maintain a flexible and balanced control of FNR and RFPR. In this article, the authors illustrate how to maintain a balanced control of both FNR and RFPR using the plot of error rate versus SSMD as well as how to keep high powers using the plot of power versus SSMD in RNAi high-throughput screening experiments. There are relationships among FNR, RFPR, Type I and II errors, and power. Understanding the differences and links among these concepts is essential for people to use statistical terminology correctly and effectively for data analysis in genome-scale RNAi screens. Here the authors explore these differences and links. © 2009 Society for Biomolecular Sciences.
KeywordFalse-negative rate Power Restricted false-positive rate Strictly standardized mean difference Type I error Type II error
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationMerck Research Laboratories
Recommended Citation
GB/T 7714
Zhang X.D.,Marine S.D.,Ferrer M.. Error rates and powers in genome-scale RNAi screens[J]. Journal of Biomolecular Screening,2009,14(3):230-238.
APA Zhang X.D.,Marine S.D.,&Ferrer M..(2009).Error rates and powers in genome-scale RNAi screens.Journal of Biomolecular Screening,14(3),230-238.
MLA Zhang X.D.,et al."Error rates and powers in genome-scale RNAi screens".Journal of Biomolecular Screening 14.3(2009):230-238.
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