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Evaluation of affinity interaction between small molecules and platelets by open tubular affinity capillary electrochromatography
Wang F.-Q.2; Zhang Q.2; Li C.-H.2; Wang Y.-Z.2; Hu Y.-J.1; Zhang Q.-H.2; Xia Z.-N.2; Yang F.-Q.2
2016-03-01
Source PublicationElectrophoresis
ISSN15222683 01730835
Volume37Issue:5-6Pages:736-743
Other Abstract

In this paper, an open tubular affinity capillary electrochromatography (OT-ACEC) was developed by physical adsorption of rabbit platelets on the inner surface of capillary. The interactions between small molecules include adenosine diphosphate (ADP) (positive control), protocatechuic acid (negative control) and seven natural products (salvianolic acid B, salvianic acid A sodium, hydroxysafflor yellow A, ferulic acid, chlorogenic acid, sinapic acid, caffeic acid) and platelets were evaluated by their retention factors and binding constants obtained based on peak-shift assay. Then, the activities of anti-platelet aggregation induced by thrombin (THR), ADP and arachidonic acid (AA) for those small molecules (except ADP) were evaluated by turbidimetric method. The results indicate that: (i) ADP, a platelet aggregation inducer, had strong interaction with platelet, while protocatechuic acid that had no inhibition on platelet aggregation behaved no specific interaction; (ii) there was a positive correlation between the anti-platelet aggregation activities of small molecules and their interactions with platelet, generally those compounds with higher binding constants with platelet exhibited higher activities. Therefore, the OT-ACEC method developed in the present study can be a potential method to evaluate affinity interactions between small molecules and platelets, so as to predict the biological activities such as anti-platelet aggregation for the small molecules.

KeywordAffinity Interaction Anti-platelet Aggregation Activity Binding Constant Open Tubular Affinity Capillary Electrochromatography Retention Factor
DOI10.1002/elps.201500414
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytica
WOS IDWOS:000372947700007
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang F.-Q.
Affiliation1.State Key Laboratory of QualityResearch in Chinese Medicine,Institute of Chinese MedicalSciences, University of Macau,Macao, P. R. China
2.School of Chemistry andChemical Engineering,Chongqing University,Chongqing, P. R. China
Recommended Citation
GB/T 7714
Wang F.-Q.,Zhang Q.,Li C.-H.,et al. Evaluation of affinity interaction between small molecules and platelets by open tubular affinity capillary electrochromatography[J]. Electrophoresis,2016,37(5-6):736-743.
APA Wang F.-Q.,Zhang Q.,Li C.-H.,Wang Y.-Z.,Hu Y.-J.,Zhang Q.-H.,Xia Z.-N.,&Yang F.-Q..(2016).Evaluation of affinity interaction between small molecules and platelets by open tubular affinity capillary electrochromatography.Electrophoresis,37(5-6),736-743.
MLA Wang F.-Q.,et al."Evaluation of affinity interaction between small molecules and platelets by open tubular affinity capillary electrochromatography".Electrophoresis 37.5-6(2016):736-743.
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