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Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis
Qian Zhang2; Cen Chen2; Feng-Qin Wang2; Chun-Hong Li2; Qi-Hui Zhang2; Yuan-Jia Hu1; Zhi-Ning Xia2; Feng-Qing Yang2
2016-12-01
Source PublicationPharmaceutical Biology
ISSN17445116 13880209
Volume54Issue:12Pages:3113-3120
Abstract

Context: The rising problem of atherosclerosis and ischemic heart disease emphasizes the need to look for new antithrombotic components with effective modes of action. Corydalis yanhusuo (Y.H. Chou & Chun C. Hsu) W.T. Wang ex Z.Y. Su & C.Y. Wu (Papaveraceae) (Rhizoma Corydalis) has been used in the traditional medicines for the treatment of cardiovascular disease. Objective: The antiplatelet aggregation compounds in Rhizoma Corydalis were screened to validate its traditional medicinal use. Material and methods: Total alkaloid extract (TAE) of Rhizoma Corydalis was obtained by refluxing 100 g Rhizoma Corydalis powder with 600 mL 70% ethanol, and purified by acidification (20% HCl) and alkalization (5 M NaOH) process. Potential antiplatelet aggregation compounds in TAE were screened by a method involving platelet bio-specific extraction and HPLC-DAD/LC–MS analysis. Further in vitro antiplatelet aggregation activity confirmation of TAE and seven main alkaloids were achieved by turbidimetry method within 3 h after blood collection from rabbit carotid artery, and all the test drugs were at the concentration range of 25–350 μg/mL. Finally, HPLC-DAD was employed for the quantitative determination of seven main components in TAE. Results: Five alkaloids, identified as glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline, can be specifically extracted with platelets. The results indicated that all these five alkaloids can inhibit thrombin-induced platelet aggregation in a low dose (IC of glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline were 49.057, 34.914, 33.547, 84.261 and 54.164 μg/mL, respectively) as compared to TAE (IC=175.426 μg/mL) and aspirin (IC=300.340 μg/mL), while the unbound compounds (palmatine and tetrahydropalmatine) had a very weak antiplatelet effect (IC>200 μg/mL). Discussion and conclusion: This study is the first reported work for antiplatelet components screening in Rhizoma Corydalis. Seven compounds were detected and identified by HPLC-DAD/LC–MS, of which five platelet-targeted compounds were discovered.

KeywordHplc/lc–ms Platelet Bio-specific Extraction Thromboembolic Disease Traditional Chinese Medicines
DOI10.1080/13880209.2016.1211714
URLView the original
Language英语
WOS Research AreaPlant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS IDWOS:000389443300042
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorFeng-Qing Yang
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P.R. China
2.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P.R. China
Recommended Citation
GB/T 7714
Qian Zhang,Cen Chen,Feng-Qin Wang,et al. Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis[J]. Pharmaceutical Biology,2016,54(12):3113-3120.
APA Qian Zhang.,Cen Chen.,Feng-Qin Wang.,Chun-Hong Li.,Qi-Hui Zhang.,...&Feng-Qing Yang.(2016).Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis.Pharmaceutical Biology,54(12),3113-3120.
MLA Qian Zhang,et al."Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis".Pharmaceutical Biology 54.12(2016):3113-3120.
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