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Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats
Li W.2; Zhao W.3; Wu Q.2; Lu Y.2; Shi J.2; Chen X.3
2016
Source PublicationJournal of Diabetes Research
ISSN23146753 23146745
Volume2016
Abstract

Objective. Puerarin is a natural flavonoid isolated from the TCM lobed kudzuvine root. This study investigated the effect and mechanisms of puerarin on diabetic aorta in rats. Methods. Streptozotocin- (STZ-) induced diabetic rats were administered with puerarin for 3 weeks. Levels of serum insulin (INS), PGE2, endothelin (ET), glycated hemoglobin (GHb), HO, and nitric oxide (NO) in rats were measured by ELISA and colorimetric assay kits. The aortas were stained with H&E. Moreover, the mRNA expression of ICAM-1, LOX-1, NADPH oxidase 2 (NOX2), and NOX4 and the protein expression of ICAM-1, LOX-1, NF-B p65, E-selectin, NOX2, and NOX4 in aorta tissues were measured by real-time PCR and Western blot, respectively. The localization of ICAM-1, NF-B p65, NOX2, and NOX4 in the aorta tissues was also determined through immunohistochemistry. Results. Puerarin treatment exerted no effect on fasting blood glucose levels but significantly reduced the serum levels of INS, GHb, PGE2, ET, HO, and NO. In addition, puerarin improved the pathological alterations and inhibited the expression of ICAM-1, LOX-1, NOX2, and NOX4 at both mRNA and protein levels. Puerarin also significantly reduced the number of cells showing positive staining for ICAM-1, NOX2, NOX4, and NF-B p65. Conclusion. Puerarin demonstrated protective effect on the STZ-induced diabetic rat aorta. The protective mechanisms may include regulation of NF-B and inhibition of NOX2 and NOX4 followed by inhibition of cell adhesion molecule expression.

DOI10.1155/2016/8541520
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism ; Research & Experimental Medicine
WOS SubjectEndocrinology & Metabolism ; Medicine, Research & Experimental
WOS IDWOS:000372991600001
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Chengdu Chronic Diseases Hospital
2.Zunyi Medical College
3.Universidade de Macau
Recommended Citation
GB/T 7714
Li W.,Zhao W.,Wu Q.,et al. Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats[J]. Journal of Diabetes Research,2016,2016.
APA Li W.,Zhao W.,Wu Q.,Lu Y.,Shi J.,&Chen X..(2016).Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats.Journal of Diabetes Research,2016.
MLA Li W.,et al."Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats".Journal of Diabetes Research 2016(2016).
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