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A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture
Beers J.2; Linask K.L.2; Chen J.A.2; Siniscalchi L.I.2; Lin Y.2; Zheng W.1; Rao M.4; Chen G.2
2015-06-11
Source PublicationScientific Reports
ISSN20452322
Volume5
Abstract

Factors limiting the adoption of iPSC technology include the cost of developing lines and the time period that it takes to characterize and bank them, particularly when integration free, feeder free, and Xeno-free components are used. In this manuscript we describe our optimization procedure that enables a single technician to make 20-40 lines at a time in a 24-96 well format in a reliable and reproducible fashion. Improvements spanned the entire workflow and included using RNA virus, reducing cytotoxicity of reagents, developing improved transfection and freezing efficiencies, modifying the manual colony picking steps, enhancing passaging efficiency and developing early criteria of success. These modifications allowed us to make more than two hundred well-characterized lines per year.

DOI10.1038/srep11319
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000356100000001
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Cited Times [WOS]:66   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.National Center for Advancing Translational Sciences
2.National Heart, Lung, and Blood Institute
3.Universidade de Macau
4.National Institutes of Health, Bethesda
Recommended Citation
GB/T 7714
Beers J.,Linask K.L.,Chen J.A.,et al. A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture[J]. Scientific Reports,2015,5.
APA Beers J.,Linask K.L.,Chen J.A.,Siniscalchi L.I.,Lin Y.,Zheng W.,Rao M.,&Chen G..(2015).A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture.Scientific Reports,5.
MLA Beers J.,et al."A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture".Scientific Reports 5(2015).
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