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A novel high-throughput technique for identifying monoclonal antibodies capable of death receptor induced apoptosis
Kwok H.F.1; Gormley J.A.1; Scott C.J.2; Johnston J.A.1; Olwill S.A.1
2009-12-01
Source PublicationJournal of Cell Death
ISSN11790660
Volume2009Pages:45-51
AbstractThe study of death receptor family induced apoptosis has gained momentum in recent years with the knowledge that therapeutic antibodies targeting DR4 and DR5 (death receptor's 4 and 5) have proved efficacious in multiple clinical trials. The therapeutic rationale is based on targeting and amplifying a tumour tissues normal cell death programme (apoptosis). While advances in the targeting of DR4 and DR5 have been successful the search for an agonistic antibody to another family member, the Fas receptor, has proven more elusive. This is partly due to the differing in vitro and in vivo characteristics of individual antibodies. In order to induce Fas targeted cell death an antibody must be capable of binding to and trimerising the receptor. It has been shown that antibodies capable of performing this function in vivo, with the assistance of tumour associated cells, do not always induce apoptosis in vitro. As a result the use of current methodologies to detect functional antibodies in vitro may have dismissed potential therapeutic candidates ('false negative'). Here we report a novel high throughput screening technique which artificially cross-links antibodies bound to the Fas receptor. By combining this process with Annexin-V and Prodidium Iodide (PI) staining we can select for antibodies which have the potential to induce apoptosis in vivo. © the authers.
KeywordAnnexin-V Apoptosis Crosslinks antibodies Fas receptor High throughput screening Prodidium iodide Therapeutic antibodies
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Fusion Antibodies plc
2.Queen's University Belfast
Recommended Citation
GB/T 7714
Kwok H.F.,Gormley J.A.,Scott C.J.,et al. A novel high-throughput technique for identifying monoclonal antibodies capable of death receptor induced apoptosis[J]. Journal of Cell Death,2009,2009:45-51.
APA Kwok H.F.,Gormley J.A.,Scott C.J.,Johnston J.A.,&Olwill S.A..(2009).A novel high-throughput technique for identifying monoclonal antibodies capable of death receptor induced apoptosis.Journal of Cell Death,2009,45-51.
MLA Kwok H.F.,et al."A novel high-throughput technique for identifying monoclonal antibodies capable of death receptor induced apoptosis".Journal of Cell Death 2009(2009):45-51.
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