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Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
Shuralev E.1; Quinn P.1; Doyle M.3; Duignan A.; Kwok H.F.2; Bezos J.5; Olwill S.A.2; Gormley E.3; Aranaz A.5; Good M.; Davis W.C.4; Clarke J.1; Whelan C.1
2012-01-27
Source PublicationVeterinary Microbiology
ISSN03781135 18732542
Volume154Issue:3-4Pages:292-297
Abstract

A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats. © 2011 Elsevier B.V.

KeywordAntigens Goats Multiplex Mycobacterium Bovis Serology Tuberculosis
DOI10.1016/j.vetmic.2011.07.028
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Veterinary Sciences
WOS SubjectMicrobiology ; Veterinary Sciences
WOS IDWOS:000299582000010
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Enfer Scientific
2.Fusion Antibodies plc
3.University College Dublin
4.Washington State University Pullman
5.Universidad Complutense de Madrid
Recommended Citation
GB/T 7714
Shuralev E.,Quinn P.,Doyle M.,et al. Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats[J]. Veterinary Microbiology,2012,154(3-4):292-297.
APA Shuralev E..,Quinn P..,Doyle M..,Duignan A..,Kwok H.F..,...&Whelan C..(2012).Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats.Veterinary Microbiology,154(3-4),292-297.
MLA Shuralev E.,et al."Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats".Veterinary Microbiology 154.3-4(2012):292-297.
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