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High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis
Kam R.K.T.; Poon T.C.W.; Chan H.L.Y.; Wong N.; Hui A.Y.; Sung J.J.Y.
2007-07-01
Source PublicationClinical Chemistry
ISSN00099147
Volume53Issue:7Pages:1254-1263
Abstract

Background: The use of MALDI-TOF mass spectrometry (MS) in quantitative glycan profiling has not been reported. In this study, we attempted to establish a high-throughput quantitative assay for profiling serum N-glycome, and we applied the new assay to identifying serum N-glycans for diagnosis of liver fibrosis and cirrhosis. Methods: N-glycans from whole serum proteins in 2 μL serum were released by enzymatic digestion, cleaned up by hydrophilic chromatography, and subsequently quantitatively profiled with a linear MALDI-TOF MS system, which was originally designed for quantitative proteomic profiling. Serum N-glycome profiles from 46 patients with chronic hepatitis B infection and with different degrees of liver fibrosis were examined. Results: The intra- and interassay CVs of peak intensities of the standard N-glycans were <8% and <17%, respectively. When the assay was applied to the analysis of serum N-glycome profiles, 17 peaks were found to be potential biomarkers for detection of liver fibrosis/ cirrhosis. Linear regression analysis revealed that 4 peaks of 1341.5, 1829.7, 1933.3, and 2130.3 m/z (all P <0.005) had complementary value in detecting liver fibrosis and included them, but not any serological markers, in the diagnostic model. Leave-one-out cross-validation showed the diagnostic model could identify significant fibrosis (Ishak score ≥3) and cirrhosis (Ishak score ≥5), both at 85% accuracy. Conclusion: This is the first study to illustrate the quantitative aspect of MALDI-TOF MS in N-glycome profiling and the first study to reveal the potential value of the serum N-glycan profile for identifying liver fibrosis. © 2007 American Association for Clinical Chemistry.

DOI10.1373/clinchem.2007.085563
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaMedical Laboratory Technology
WOS SubjectMedical Laboratory Technology
WOS IDWOS:000247558000013
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Cited Times [WOS]:41   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorPoon T.C.W.
AffiliationPrince of Wales Hospital Hong Kong
Recommended Citation
GB/T 7714
Kam R.K.T.,Poon T.C.W.,Chan H.L.Y.,et al. High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis[J]. Clinical Chemistry,2007,53(7):1254-1263.
APA Kam R.K.T.,Poon T.C.W.,Chan H.L.Y.,Wong N.,Hui A.Y.,&Sung J.J.Y..(2007).High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis.Clinical Chemistry,53(7),1254-1263.
MLA Kam R.K.T.,et al."High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis".Clinical Chemistry 53.7(2007):1254-1263.
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