UM  > Faculty of Health Sciences
Radiation-induced genomic instability in Caenorhabditis elegans
Huumonen K.1; Immonen H.-K.1; Baverstock K.1; Hiltunen M.1; Korkalainen M.2; Lahtinen T.3; Parviainen J.1; Viluksela M.2; Wong G.1; Naarala J.1; Juutilainen J.1
2012-10-09
Source PublicationMutation Research - Genetic Toxicology and Environmental Mutagenesis
ISSN13835718 18793592
Volume748Issue:1-2Pages:36-41
Abstract

Radiation-induced genomic instability has been well documented, particularly in vitro. However, the understanding of its mechanisms and their consequences in vivo is still limited. In this study, Caenorhabditis elegans (C. elegans; strain CB665) nematodes were exposed to X-rays at doses of 0.1, 1, 3 or 10. Gy. The endpoints were measured several generations after exposure and included mutations in the movement-related gene unc-58, alterations in gene expression analysed with oligoarrays containing the entire C. elegans genome, and micro-satellite mutations measured by capillary electrophoresis. The progeny of the irradiated nematodes showed an increased mutation frequency in the unc-58 gene, with a maximum response observed at 1. Gy. Significant differences were also found in gene expression between the irradiated (1. Gy) and non-irradiated nematode lines. Differences in gene expression did not show clear clustering into certain gene categories, suggesting that the instability might be a chaotic process rather than a result of changes in the function of few specific genes such as, e.g., those responsible for DNA repair. Increased heterogeneity in gene expression, which has previously been described in irradiated cultured human lymphocytes, was also observed in the present study in C. elegans, the coefficient of variation of gene expression being higher in the progeny of irradiated nematodes than in control nematodes. To the best of our knowledge, this is the first publication reporting radiation-induced genomic instability in C. elegans. © 2012 Elsevier B.V.

KeywordDose-response Ionizing Radiation Transgenerational Effects
DOI10.1016/j.mrgentox.2012.06.010
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS IDWOS:000308515300006
Fulltext Access
Citation statistics
Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Itä-Suomen yliopisto
2.National Institute for Health and Welfare
3.Kuopio University Hospital
Recommended Citation
GB/T 7714
Huumonen K.,Immonen H.-K.,Baverstock K.,et al. Radiation-induced genomic instability in Caenorhabditis elegans[J]. Mutation Research - Genetic Toxicology and Environmental Mutagenesis,2012,748(1-2):36-41.
APA Huumonen K.,Immonen H.-K.,Baverstock K.,Hiltunen M.,Korkalainen M.,Lahtinen T.,Parviainen J.,Viluksela M.,Wong G.,Naarala J.,&Juutilainen J..(2012).Radiation-induced genomic instability in Caenorhabditis elegans.Mutation Research - Genetic Toxicology and Environmental Mutagenesis,748(1-2),36-41.
MLA Huumonen K.,et al."Radiation-induced genomic instability in Caenorhabditis elegans".Mutation Research - Genetic Toxicology and Environmental Mutagenesis 748.1-2(2012):36-41.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huumonen K.]'s Articles
[Immonen H.-K.]'s Articles
[Baverstock K.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huumonen K.]'s Articles
[Immonen H.-K.]'s Articles
[Baverstock K.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huumonen K.]'s Articles
[Immonen H.-K.]'s Articles
[Baverstock K.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.