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Regulation of the localization and stability of Cdc6 in living yeast cells
Luo K.Q.1; Elsasser S.1; Chang D.C.2; Campbell J.L.1
2003-07-11
Source PublicationBiochemical and Biophysical Research Communications
ISSN0006291X
Volume306Issue:4Pages:851-859
Abstract

The Cdc6 protein is an essential regulator for initiation of DNA replication. Following the G1/S transition, Cdc6 is degraded through a ubiquitin-mediated proteolysis pathway. In this study, we tagged Cdc6 with green fluorescent protein (GFP) and used site-specific mutations to study the regulation of Cdc6 localization and degradation in living yeast cells. Our major findings are: (1) Cdc6-GFP distributes predominantly in the nucleus in all cell cycle stages, with a small increase in cytoplasmic localization in G2/M cells. (2) This nuclear localization is critical for Cdc6 degradation. When the N-terminal nuclear localization signal (NLS) was mutated, Cdc6-GFP no longer accumulated in the nucleus, and the mutant cdc6 was stabilized compared to wild type. (3) The putative CDK phosphorylation sites are not required for Cdc6 nuclear localization, but are important for protein stability. These observations suggest that the stability of Cdc6 protein is regulated by two factors: nuclear localization and phosphorylation by CDK1. © 2003 Elsevier Science (USA). All rights reserved.

KeywordCdc6 G1/s Transition Gfp Nuclear Localization And Phosphorylation Yeast
DOI10.1016/S0006-291X(03)01082-9
URLView the original
Indexed BySCIE
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000183992400009
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.California Institute of Technology
2.Hong Kong University of Science and Technology
Recommended Citation
GB/T 7714
Luo K.Q.,Elsasser S.,Chang D.C.,et al. Regulation of the localization and stability of Cdc6 in living yeast cells[J]. Biochemical and Biophysical Research Communications,2003,306(4):851-859.
APA Luo K.Q.,Elsasser S.,Chang D.C.,&Campbell J.L..(2003).Regulation of the localization and stability of Cdc6 in living yeast cells.Biochemical and Biophysical Research Communications,306(4),851-859.
MLA Luo K.Q.,et al."Regulation of the localization and stability of Cdc6 in living yeast cells".Biochemical and Biophysical Research Communications 306.4(2003):851-859.
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