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Smac/DIABLO and cytochrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis
Zhou L.L.; Zhou L.Y.; Luo K.Q.; Chang D.C.
2005-03-01
Source PublicationApoptosis
ISSN13608185
Volume10Issue:2Pages:289-299
Abstract

During apoptosis, a key event is the release of Smac/DIABLO (an inhibitor of XIAP) and cytochrome c (Cyt-c, an activator of caspase-9) from mitochondria to cytosol. It was not clear, however, whether the releasing mechanisms of these two proteins are the same. Using a combination of single living-cell analysis and immunostaining techniques, we investigated the dynamic process of Smac and Cyt-c release during UV-induced apoptosis in HeLa cells. We found that YFP-labeled Smac and GFP-labeled Cyt-c were released from mitochondria in the same time window, which coincided with the mitochondrial membrane potential depolarization. Furthermore, using immunostaining, we found that the endogenous Smac and Cyt-c were always released together within an individual cell. Finally, when cells were pre-treated with caspase inhibitor (z-VAD-fmk) to block caspase activation, the process of Smac release, like that of Cyt-c, was not affected. This was true for both YFP-labeled Smac and endogenous Smac. These results suggest that in HeLa cells, both Smac and Cyt-c are released from mitochondria during UV-induced apoptosis through the same permeability transition mechanism, which we believe is triggered by the aggregation of Bax in the outer mitochondrial membrane to form lipid-protein complex. © 2005 Springer Science + Business Media, Inc.

KeywordApoptosis Caspase Cytochrome c Mitochondria Smac/diablo
DOI10.1007/s10495-005-0803-9
URLView the original
Indexed BySCIE
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000228639600004
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Cited Times [WOS]:43   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationHong Kong University of Science and Technology
Recommended Citation
GB/T 7714
Zhou L.L.,Zhou L.Y.,Luo K.Q.,et al. Smac/DIABLO and cytochrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis[J]. Apoptosis,2005,10(2):289-299.
APA Zhou L.L.,Zhou L.Y.,Luo K.Q.,&Chang D.C..(2005).Smac/DIABLO and cytochrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis.Apoptosis,10(2),289-299.
MLA Zhou L.L.,et al."Smac/DIABLO and cytochrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis".Apoptosis 10.2(2005):289-299.
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