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An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE
Yu Y.1; Qin A.1; Feng C.1; Lu P.3; Ng K.M.1; Luo K.Q.2; Tang B.Z.1
2012
Source PublicationAnalyst
ISSN13645528 00032654
Volume137Issue:23Pages:5592-5596
Abstract

A new aggregation-induced emission (AIE) compound 1,2-bis[4- (isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved. © 2012 The Royal Society of Chemistry.

DOI10.1039/c2an36002k
URLView the original
Indexed BySCIE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000310375200022
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Hong Kong University of Science and Technology
2.Nanyang Technological University
3.Jilin University
Recommended Citation
GB/T 7714
Yu Y.,Qin A.,Feng C.,et al. An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE[J]. Analyst,2012,137(23):5592-5596.
APA Yu Y.,Qin A.,Feng C.,Lu P.,Ng K.M.,Luo K.Q.,&Tang B.Z..(2012).An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE.Analyst,137(23),5592-5596.
MLA Yu Y.,et al."An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE".Analyst 137.23(2012):5592-5596.
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