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An oestrogen-receptor-α-bound human chromatin interactome
Fullwood M.J.5; Liu M.H.5; Pan Y.F.5; Liu J.5; Xu H.5; Mohamed Y.B.5; Orlov Y.L.5; Velkov S.5; Ho A.5; Mei P.H.5; Chew E.G.Y.5; Huang P.Y.H.5; Welboren W.-J.1; Han Y.5; Ooi H.S.5; Ariyaratne P.N.5; Vega V.B.5; Luo Y.5; Tan P.Y.5; Choy P.Y.5; Wansa K.D.S.A.5; Zhao B.5; Lim K.S.5; Leow S.C.5; Yow J.S.5; Joseph R.5; Li H.5; Desai K.V.5; Thomsen J.S.5; Lee Y.K.5; Karuturi R.K.M.5; Herve T.5; Bourque G.5; Stunnenberg H.G.1; Ruan X.5; Cacheux-Rataboul V.5; Sung W.-K.5; Liu E.T.5; Wei C.-L.5; Cheung E.5; Ruan Y.5
Source PublicationNature
ISSN00280836 14764687

Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor α (ER-α) in the human genome. We found that most high-confidence remote ER-α-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-α functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes. © 2009 Macmillan Publishers Limited. All rights reserved.

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Indexed BySCI
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000271419200031
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Cited Times [WOS]:1000   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Nijmegen Centre for Molecular Life Sciences - NCMLS
2.Nanyang Technological University
3.National University of Singapore
4.Yong Loo Lin School of Medicine
5.A-Star, Genome Institute of Singapore
Recommended Citation
GB/T 7714
Fullwood M.J.,Liu M.H.,Pan Y.F.,et al. An oestrogen-receptor-α-bound human chromatin interactome[J]. Nature,2009,462(7269):58-64.
APA Fullwood M.J..,Liu M.H..,Pan Y.F..,Liu J..,Xu H..,...&Ruan Y..(2009).An oestrogen-receptor-α-bound human chromatin interactome.Nature,462(7269),58-64.
MLA Fullwood M.J.,et al."An oestrogen-receptor-α-bound human chromatin interactome".Nature 462.7269(2009):58-64.
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