UM
Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development
Ito Y.1; Sarkar P.2; Mi Q.1; Wu N.1; Bringas Jr. P.1; Liu Y.1; Reddy S.2; Maxson R.1; Deng C.3; Chai Y.1
2001-08-01
Source PublicationDevelopmental Biology
ISSN00121606
Volume236Issue:1Pages:230-243
AbstractActivation of the Xenopus bone morphogenetic protein (BMP) pathway is coincident with the onset of zygotic transcription but requires maternal signaling proteins. The mechanisms controlling the translation of mRNAs that encode proteins of the BMP pathway were investigated by using polysome association as an assay for translational activity. Our results indicate that five different mRNAs encoding proteins of the BMP pathway were translationally regulated during Xenopus development. These mRNAs were either not associated or inefficiently associated with polysomes in oocytes, and each was recruited to polysomes at a different developmental stage. The Smad1 and ALK-2 mRNAs were recruited to polysomes during oocyte maturation, whereas the BMP-7 and XSTK9 mRNAs were recruited during the early stages of embryogenesis. The ALK-3 mRNA was not efficiently associated with polysomes during any maternal stage of development and was efficiently recruited to polysomes only after the onset of zygotic transcription. In general, for all stages except oocytes, polysome recruitment was associated with the presence of a 3′ poly(A) tail. However, there was not an obvious correlation between the absolute length of poly(A) and the efficiency of polysome recruitment, indicating that the relationship between poly(A) tail length and translation during early frog embryogenesis is complex. We further focused on the BMP-7 mRNA and demonstrated that sequence elements within the 3′UTR were necessary for recruitment of the BMP-7 mRNA to polysomes and sufficient to direct the addition of poly(A) and activate translation of a reporter during embryogenesis. Interestingly, the BMP-7 mRNA lacks the previously defined eCPE sequences proposed to direct poly(A) addition and translational activation during embryogenesis. The implications of our findings for translational regulation of maternal mRNAs during embryo-genesis and for the activation of the BMP pathway are discussed. © 2001 Academic Press.
KeywordBMP signaling Translational control Xenopus embryogenesis
DOI10.1006/dbio.2001.0324
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Southern California
2.Keck School of Medicine of USC
3.National Institute of Diabetes and Digestive and Kidney Diseases
Recommended Citation
GB/T 7714
Ito Y.,Sarkar P.,Mi Q.,et al. Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development[J]. Developmental Biology,2001,236(1):230-243.
APA Ito Y.,Sarkar P.,Mi Q.,Wu N.,Bringas Jr. P.,Liu Y.,Reddy S.,Maxson R.,Deng C.,&Chai Y..(2001).Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development.Developmental Biology,236(1),230-243.
MLA Ito Y.,et al."Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development".Developmental Biology 236.1(2001):230-243.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ito Y.]'s Articles
[Sarkar P.]'s Articles
[Mi Q.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ito Y.]'s Articles
[Sarkar P.]'s Articles
[Mi Q.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ito Y.]'s Articles
[Sarkar P.]'s Articles
[Mi Q.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.