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Transcriptomic analysis for differentially expressed genes in ovarian follicle activation in the zebrafish
Bo Zhu1; Lakhansing Pardeshi.2; Yingying Chen2; Wei Ge1
2018
Source PublicationFrontiers in Endocrinology
ISSN1664-2392
Volume9Issue:593
Abstract

In teleosts, the onset of puberty in females is marked by the appearance of the first wave of pre-vitellogenic (PV) follicles from the pool of primary growth (PG) follicles (follicle activation) in the ovary during sexual maturation. To understand the mechanisms underlying follicle activation and therefore puberty onset, we undertook this transcriptomic study to investigate gene expression profiles in the event. Our analysis revealed a total of 2,027 up-regulated and 859 down-regulated genes during the PG-PV transition. Gene Ontology (GO) analysis showed that in addition to basic cellular functions such as gene transcription, cell differentiation, and cell migration, other biological processes such as steroidogenesis, cell signaling and angiogenesis were also enriched in up-regulated genes; by comparison, some processes were down-regulated including piRNA metabolism, gene silencing and proteolysis. Further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified a variety of signaling pathways that might play pivotal roles in PG-PV transition, including MAPK, TGF-β, Hedgehog, FoxO, VEGF, Jak-STAT, and phosphatidylinositol signaling pathways. Other pathways of particular interest included endocytosis and glycosaminoglycan biosynthesis. We also analyzed expression changes of genes expressed in different compartments viz. oocytes and follicle cells. Interestingly, most oocyte-specific genes remained unchanged in expression during follicle activation whereas a great number of genes specifically expressed in the follicle cells showed significant changes in expression. Overall, this study reported a comprehensive analysis for genes, biological processes and pathways involved in follicle activation, which also marks female puberty onset in the zebrafish when occurring for the first time in sexual maturation. Copyright © 2018 Zhu, Pardeshi, Chen and Ge. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

KeywordFollicles Folliculogenesis Puberty Rna-seq Transcriptomics Zebrafish
DOI10.3389/fendo.2018.00593
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000446996200001
The Source to ArticleScopus
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Taipa, China
2.Genomics and Bioinformatics Core, Faculty of Health Sciences, University of Macau, Taipa, China
First Author AffilicationCentre of Reproduction, Development and Aging
Recommended Citation
GB/T 7714
Bo Zhu,Lakhansing Pardeshi.,Yingying Chen,et al. Transcriptomic analysis for differentially expressed genes in ovarian follicle activation in the zebrafish[J]. Frontiers in Endocrinology,2018,9(593).
APA Bo Zhu,Lakhansing Pardeshi.,Yingying Chen,&Wei Ge.(2018).Transcriptomic analysis for differentially expressed genes in ovarian follicle activation in the zebrafish.Frontiers in Endocrinology,9(593).
MLA Bo Zhu,et al."Transcriptomic analysis for differentially expressed genes in ovarian follicle activation in the zebrafish".Frontiers in Endocrinology 9.593(2018).
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