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Differential regulation of kit ligand A (kitlga) expression in the zebrafish ovarian follicle cells - Evidence for the existence of a cyclic adenosine 3 ', 5 ' monophosphate-mediated binary regulatory system during folliculogenesis
Yao, Kai1; Ge, Wei2,3
2015-02-15
Source PublicationMOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN0303-7207
Volume402Issue:CPages:21-31
Abstract

Kit ligand (Kitl) is an important paracrine factor involved in the activation of primordial follicles from the quiescent pool and in the maintenance of meiotic arrest before germinal vesicle breakdown (GVBD). It has been reported that follicle-stimulating hormone (FSH) stimulates but luteinizing hormone (LH) suppresses the expression of Kitl in the granulosa cells in mammals. Considering that both gonadotropins signal in the follicle cells mainly by activating cyclic adenosine 3', 5'-monophosphate (cAMP) pathway, we are intrigued by how cAMP differentially regulates Kitl expression. In the present study, we demonstrated that both human chorionic gonadotropin (hCG) and pituitary adenylate cyclase activating polypeptide (PACAP) inhibited insulin-like growth factor I (IGF-I)-induced Akt phosphorylation and kitlga expression in the zebrafish follicle cells. Further experiments showed that cAMP was involved in regulating the expression of kitlga. However, two cAMP-activated effectors, protein kinase A (PICA) and exchange protein directly activated by CAMP (Epac), had converse effects. PICA promoted whereas Epac inhibited the expression of kitlga, as demonstrated by the respective activators. Interestingly, cAMP also appeared to exert differential effects on kitlga expression at different stages of follicle development during folliculogenesis, significantly stimulating kitlga expression at the early growth stage but suppressing it at the full-grown stage before final oocyte maturation, implying a potential mechanism for differential effects of the same pathway at different stages. The inhibitory effect of forskolin (activator of adenylate cyclase) and H89 (inhibitor of PKA) on IGF-I-induced expression of kitlga suggested cross-talk between the cAMP and IGF-I-activated PI3K-Akt pathways. This study, together with our previous findings on IGF-I regulation of kitlga expression, provides important clues to the underlying mechanism that regulates Kit ligand expression during folliculogenesis in the ovary. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

KeywordKit Ligand a Camp Pka Epac Ovary Zebrafish
DOI10.1016/j.mce.2014.12.005
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaCell Biology ; Endocrinology & Metabolism
WOS SubjectCell Biology ; Endocrinology & Metabolism
WOS IDWOS:000350093800003
PublisherELSEVIER IRELAND LTD
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorGe, Wei
Affiliation1.School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
2.School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
3.Faculty of Health Sciences, University of Macau, Taipa, Macau, China
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Yao, Kai,Ge, Wei. Differential regulation of kit ligand A (kitlga) expression in the zebrafish ovarian follicle cells - Evidence for the existence of a cyclic adenosine 3 ', 5 ' monophosphate-mediated binary regulatory system during folliculogenesis[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2015,402(C):21-31.
APA Yao, Kai,&Ge, Wei.(2015).Differential regulation of kit ligand A (kitlga) expression in the zebrafish ovarian follicle cells - Evidence for the existence of a cyclic adenosine 3 ', 5 ' monophosphate-mediated binary regulatory system during folliculogenesis.MOLECULAR AND CELLULAR ENDOCRINOLOGY,402(C),21-31.
MLA Yao, Kai,et al."Differential regulation of kit ligand A (kitlga) expression in the zebrafish ovarian follicle cells - Evidence for the existence of a cyclic adenosine 3 ', 5 ' monophosphate-mediated binary regulatory system during folliculogenesis".MOLECULAR AND CELLULAR ENDOCRINOLOGY 402.C(2015):21-31.
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