UM
Cloning and functional characterization of the mouse C3a anaphylatoxin receptor gene
Hsu M.H.; Ember J.A.; Wang M.; Prossnitz E.R.; Hugli T.E.; Ye R.D.
1997-12-01
Source PublicationImmunogenetics
ISSN00937711
Volume47Issue:1Pages:64-72
AbstractThe mouse anaphylatoxin C3a receptor (mC3aR) gene was isolated using a human C3aR cDNA probe. The genomic fragment contains an open reading frame of 1431 base pairs that encodes a peptide of 477 amino acids. A cDNA with identical sequence was subsequently obtained from the mouse pre-B cell line 70Z/3 by reverse transcriptase polymerase chain reaction based on sequence of the mC3aR gene. Northern blot analysis suggested expression of the MC3aR in lung and heart, and to a lesser extent, in brain, liver, muscle, kidney, and testis. The deduced amino acid sequence of the mouse C3aR is 65% identical to that of the human C3aR. Like the human receptor, mouse C3aR contains a predicted large extracellular loop of ~ 165 amino acids (residues 161-325) between the fourth and fifth transmembrane domains. This loop, however, is the least conserved structure (45% identical sequence) of all the extracellular and intracellular domains between the mouse and human C3aRs. The mouse gene product bound I-labeled human C3a with a K(d) of 2.54 nM when expressed in the stably transfected rat basophilic leukemic cell line RBL-2H3. Bound C3a could be effectively displaced by excess quantities of unlabeled C3a, but not by C4a or C5a. C3a induced dose-dependent calcium mobilization in the transfected cells, which could be blocked by pertussis toxin treatment. These results confirm that the cloned gene encodes a functional C3aR capable of coupling to a pertussis toxin-sensitive G protein. The sequence divergence of the large extracellular loop does not appear to affect C3a binding and transmembrane signaling.
KeywordAnaphylatoxin C3a Chemoattractant Complement G protein-coupled receptor
DOI10.1007/s002510050327
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationScripps Research Institute
Recommended Citation
GB/T 7714
Hsu M.H.,Ember J.A.,Wang M.,et al. Cloning and functional characterization of the mouse C3a anaphylatoxin receptor gene[J]. Immunogenetics,1997,47(1):64-72.
APA Hsu M.H.,Ember J.A.,Wang M.,Prossnitz E.R.,Hugli T.E.,&Ye R.D..(1997).Cloning and functional characterization of the mouse C3a anaphylatoxin receptor gene.Immunogenetics,47(1),64-72.
MLA Hsu M.H.,et al."Cloning and functional characterization of the mouse C3a anaphylatoxin receptor gene".Immunogenetics 47.1(1997):64-72.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hsu M.H.]'s Articles
[Ember J.A.]'s Articles
[Wang M.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hsu M.H.]'s Articles
[Ember J.A.]'s Articles
[Wang M.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hsu M.H.]'s Articles
[Ember J.A.]'s Articles
[Wang M.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.