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Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe
Modi Wang1; Bingyong He1; Lihua Lu1; Chung-Hang Leung2; Jean-Louis Mergny3,4; Dik-Lung Ma1,5
2015-08-05
Source PublicationBiosensors and Bioelectronics
ISSN18734235 09565663
Volume70Pages:338-344
Abstract

We have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This "mix-and-detect" assay is simple and selective, and could detect down to 10nM of the target gene in aqueous solution with a linear range from 10 to 500nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.

KeywordG-quadruplex Gene Deletion Iridium(Iii) Complex Label-free Detection Luminescent Probe
DOIhttps://doi.org/10.1016/j.bios.2015.03.047
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000356554400047
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Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorJean-Louis Mergny; Dik-Lung Ma
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.ARNA Laboratory, University of Bordeaux, IECB, F-33000 Bordeaux, France
4.INSERM U869, IECB, F-33600 Pessac, France
5.Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, PR China
Recommended Citation
GB/T 7714
Modi Wang,Bingyong He,Lihua Lu,et al. Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe[J]. Biosensors and Bioelectronics,2015,70:338-344.
APA Modi Wang,Bingyong He,Lihua Lu,Chung-Hang Leung,Jean-Louis Mergny,&Dik-Lung Ma.(2015).Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe.Biosensors and Bioelectronics,70,338-344.
MLA Modi Wang,et al."Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe".Biosensors and Bioelectronics 70(2015):338-344.
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