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A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips
Chong Hu1; Sheng Lin1; Wanbo Li1; Han Sun1; Yangfan Chen2; Chiu-Wing Chan1; Chung-Hang Leung3; Dik-Lung Ma1,4; Hongkai Wu2; Kangning Ren1,2,5
2016
Source PublicationLab on a Chip
ISSN14730189 14730197
Volume16Issue:20Pages:3909-3918
Abstract

An ultra-fast, extremely cost-effective, and environmentally friendly method was developed for fabricating flexible microfluidic chips with plastic membranes. With this method, we could fabricate plastic microfluidic chips rapidly (within 12 seconds per piece) at an extremely low cost (less than $0.02 per piece). We used a heated perfluoropolymer perfluoroalkoxy (often called Teflon PFA) solid stamp to press a pile of two pieces of plastic membranes, low density polyethylene (LDPE) and polyethylene terephthalate (PET) coated with an ethylene-vinyl acetate copolymer (EVA). During the short period of contact with the heated PFA stamp, the pressed area of the membranes permanently bonded, while the LDPE membrane spontaneously rose up at the area not pressed, forming microchannels automatically. These two regions were clearly distinguishable even at the micrometer scale so we were able to fabricate microchannels with widths down to 50 microns. This method combines the two steps in the conventional strategy for microchannel fabrication, generating microchannels and sealing channels, into a single step. The production is a green process without using any solvent or generating any waste. Also, the chips showed good resistance against the absorption of Rhodamine 6G, oligonucleotides, and green fluorescent protein (GFP). We demonstrated some typical microfluidic manipulations with the flexible plastic membrane chips, including droplet formation, on-chip capillary electrophoresis, and peristaltic pumping for quantitative injection of samples and reagents. In addition, we demonstrated convenient on-chip detection of lead ions in water samples by a peristaltic-pumping design, as an example of the application of the plastic membrane chips in a resource-limited environment. Due to the high speed and low cost of the fabrication process, this single-step method will facilitate the mass production of microfluidic chips and commercialization of microfluidic technologies.

DOI10.1039/c6lc00957c
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
WOS IDWOS:000385170000005
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorDik-Lung Ma; Hongkai Wu; Kangning Ren
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
2.Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
4.HKBU Institute of Research and Continuing Education, Shenzhen, China
5.State Key Laboratory of Environmental and Biological Analysis, The Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
Recommended Citation
GB/T 7714
Chong Hu,Sheng Lin,Wanbo Li,et al. A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips[J]. Lab on a Chip,2016,16(20):3909-3918.
APA Chong Hu,Sheng Lin,Wanbo Li,Han Sun,Yangfan Chen,Chiu-Wing Chan,Chung-Hang Leung,Dik-Lung Ma,Hongkai Wu,&Kangning Ren.(2016).A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips.Lab on a Chip,16(20),3909-3918.
MLA Chong Hu,et al."A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips".Lab on a Chip 16.20(2016):3909-3918.
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