Analysis of Cordyceps by multi-column liquid chromatography
Qian Z.2; Li S.2
Source PublicationActa Pharmaceutica Sinica B
ISSN22113843 22113835
AbstractCordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion pre-column into two fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC-LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks were identified as adenosine-5′-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine, deoxyadenosine-5′-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps.
KeywordCordyceps Macromolecules Micromolecules Multi-column Size exclusion column
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Sunshine Lake Pharma Co., Ltd
2.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Qian Z.,Li S.. Analysis of Cordyceps by multi-column liquid chromatography[J]. Acta Pharmaceutica Sinica B,2017,7(2):202-207.
APA Qian Z.,&Li S..(2017).Analysis of Cordyceps by multi-column liquid chromatography.Acta Pharmaceutica Sinica B,7(2),202-207.
MLA Qian Z.,et al."Analysis of Cordyceps by multi-column liquid chromatography".Acta Pharmaceutica Sinica B 7.2(2017):202-207.
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