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Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis
Wing-Yan Li1; Yam-Fung Ng2; Huan Zhang2; Zi-Dong Guo1; De-Jian Guo1; Yiu-Wa Kwan3; George Pak-Heng Leung4; Simon Ming-Yuen Lee5; Peter Hoi-Fu Yu1,2; Shun-Wan Chan1,2
2014
Source PublicationInflammopharmacology
ISSN15685608 09254692
Volume22Issue:2Pages:127-134
Abstract

This study investigated the mechanism of the cytotoxic effect of emodin, an active anthraquinone, on human lung adenocarcinoma A549 cells. In vitro growth inhibition and suppression on colony forming were used to evaluate the effects of emodin on A549 cells. Emodin's ability in changing the expressions of apoptosis-related genes was studied by real-time RT-PCR. Emodin could significantly inhibit the growth of A549 cells with IC = 16.85 μg/ml (∼60 μM). It also concentration dependently inhibited the colony-forming ability of A549 cells with IC = 7.60 μg/ml (∼30 μM). Hallmarks of apoptosis, such as single-strand DNA breakage and DNA fragmentation, were observed in A549 cells treated with emodin. Emodin (72 h) treatment could up-regulate the gene expression of FASL (p < 0.05) and down-regulate the gene expression of C-MYC (p < 0.01), but induce no significant changes in the gene expressions of MCL1, GAPDH, BAX and CCND1. These results suggest that emodin could induce growth inhibition and apoptosis in A549 cells through modifying the extrinsic apoptotic pathways and the induction of cell cycle arrest. 

KeywordCell Cycle Arrest Dna Fragmentation Emodin Growth Inhibition
DOI10.1007/s10787-013-0186-4
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaImmunology ; Pharmacology & Pharmacy ; Toxicology
WOS SubjectImmunology ; Pharmacology & Pharmacy ; Toxicology
WOS IDWOS:000332963000007
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Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Chinese Medicine and Molecular Pharmacology
2.Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
3.School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
4.Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong, China
5.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Wing-Yan Li,Yam-Fung Ng,Huan Zhang,et al. Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis[J]. Inflammopharmacology,2014,22(2):127-134.
APA Wing-Yan Li,Yam-Fung Ng,Huan Zhang,Zi-Dong Guo,De-Jian Guo,Yiu-Wa Kwan,George Pak-Heng Leung,Simon Ming-Yuen Lee,Peter Hoi-Fu Yu,&Shun-Wan Chan.(2014).Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis.Inflammopharmacology,22(2),127-134.
MLA Wing-Yan Li,et al."Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis".Inflammopharmacology 22.2(2014):127-134.
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