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Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways
Si Wan Lei1; Guozhen Cui1,2; George Pak Heng Leung3; Sharon Chui Wah Luk1; Maggie Pui Man Hoi1; Liang Wang1; Gail B. Mahady4; Simon Ming Yuen Lee1
2015-10
Source PublicationJournal of Functional Foods
ISSN1756-4646
Volume18Pages:213-223
Abstract

Icaritin, a prenylflavonoid isolated from Herbal Epimedii, is well known for its osteoplastic and anti-tumour activities. Here, we examined the cardioprotective actions of icaritin on cardiac H9c2 cells under oxidative stress conditions induced by treatment with tert-butylhydroperoxide (t-BHP). Icaritin at a concentration range between 1 and 4 μM effectively increased cell viability and decreased lactate dehydrogenase (LDH) leakage and reactive oxygen species (ROS) release in t-BHP treated cells, reflecting its ability to reduce t-BHP-induced cell injury. Icaritin also protected cell membrane integrity by preventing the collapse of the mitochondrial membrane potential. In addition, pretreatment of NF-E2-related factor 2 (Nrf2) siRNA and Akt inhibitor abolished the cardioprotective effect of icaritin. Furthermore, Nrf2 siRNA transfection interfered with the effect of icaritin on the inhibition of Ca2+ overload, whereas Akt inhibitor reduced icaritin-induced haemo-oxygenase-1 (HO-1) expression. Collectively, these results suggest that icaritin exerted its cardioprotective effect through the Akt/Nrf2/HO-1 and calcium signalling pathways.

KeywordCalcium Ion Cardiac H9c2 Cells Icaritin Nf-e2-related Factor 2 Oxidative Stress
DOI10.1016/j.jff.2015.06.054
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaFood Science & Technology ; Nutrition & Dietetics
WOS SubjectFood Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000367757400021
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorGeorge Pak Heng Leung; Simon Ming Yuen Lee
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Department of Bioengineering, Zhuhai Campus of Zunyi Medical College, Guangdong, China
3.Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
4.Departments of Pharmacy Practice and Pan-American Health Organization/World Health Organization Collaborating Centre for Traditional Medicine, College of Pharmacy, University of Illinois at Chicago, Chicago, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Si Wan Lei,Guozhen Cui,George Pak Heng Leung,et al. Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways[J]. Journal of Functional Foods,2015,18:213-223.
APA Si Wan Lei,Guozhen Cui,George Pak Heng Leung,Sharon Chui Wah Luk,Maggie Pui Man Hoi,Liang Wang,Gail B. Mahady,&Simon Ming Yuen Lee.(2015).Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways.Journal of Functional Foods,18,213-223.
MLA Si Wan Lei,et al."Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways".Journal of Functional Foods 18(2015):213-223.
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