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Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish
Xiaoqing Miao2; Ye Li2; Xueqing Wang1; Simon Ming-Yuen Lee2; Ying Zheng2
2016-05-25
Source PublicationACS Applied Materials and Interfaces
ISSN19448252 19448244
Volume8Issue:20Pages:12620-12630
Abstract

Nanocrystals (NCs) were utilized as oral formulations in commercial products to deliver lipophilic drug, but their transport mechanisms are not fully understood. This study aimed to explore the transport mechanism of NCs using in vitro Madin-Darby canine kidney II (MDCK II) cells and in vivo larval zebrafish models. Coumarin 6 (C6) was formulated into NCs with particle size of 67.5 ± 5.2 and 190 ± 9.2 nm. In vitro studies showed that 70 nm NCs accumulated in lysosome and endoplasmic reticulum (ER) as destinations. Lipid raft pathways mediated the endocytosis, while lipid raft, ER/Golgi, and Golgi/plasma membrane pathways were involved in exocytosis and transcytosis process. However, 200 nm NCs accumulated more in a lysosome, where lipid raft pathways were also involved in the endocytosis process. In vivo studies in larval zebrafish model further confirmed that the above network plays an important role in the absorption and distribution of C6-NCs.

KeywordEpithelial Cells Nanocrystals Particle Size Transport Mechanism Zebrafish
DOI10.1021/acsami.6b01680
URLView the original
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000376825800008
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Cited Times [WOS]:26   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Xiaoqing Miao,Ye Li,Xueqing Wang,et al. Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish[J]. ACS Applied Materials and Interfaces,2016,8(20):12620-12630.
APA Xiaoqing Miao,Ye Li,Xueqing Wang,Simon Ming-Yuen Lee,&Ying Zheng.(2016).Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish.ACS Applied Materials and Interfaces,8(20),12620-12630.
MLA Xiaoqing Miao,et al."Transport Mechanism of Coumarin 6 Nanocrystals with Two Particle Sizes in MDCKII Monolayer and Larval Zebrafish".ACS Applied Materials and Interfaces 8.20(2016):12620-12630.
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