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Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography
Li, SP; Li, P; Lai, CM; Gong, YX; Kan, KKW; Dong, TTX; Tsim, KWK; Wang, YT
2004
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1036Issue:2Pages:239-243
Abstract

A simple method is described for the simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps. The samples were extracted byusing pressurised liquid extraction (PLE). The effects of experimental variables, such as solvent, temperature, static extraction time and cycles, on PLE efficiency have been studied. The results showed a strong influence of the solvent and temperature on extraction efficiency of PLE. The determination was achieved by high-performance liquid chromatography (HPLC) using a Zorbax NH2 analytical column (250 x 4.6 mm i.d., 5 mum) with diode-array detector (DAD). The automated preparation of the sample permits a very fast analysis which is an important goal for routine purpose. (C) 2004 Elsevier B.V. All rights reserved.

KeywordCordyceps Spp Pharmaceutical Analysis Ergosterol Sterols Nucleosides
DOI10.1016/j.chroma.2004.02.080
URLView the original
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000221103200020
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Cited Times [WOS]:99   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China
2.Nanjing Univ Tradit Chinese Med, Natl Stand Lab Tradit Chinese Med, Nanjing, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Li, SP,Li, P,Lai, CM,et al. Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1036(2):239-243.
APA Li, SP,Li, P,Lai, CM,Gong, YX,Kan, KKW,Dong, TTX,Tsim, KWK,&Wang, YT.(2004).Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1036(2),239-243.
MLA Li, SP,et al."Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1036.2(2004):239-243.
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