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Siegesbeckia pubescens Makino inhibits Pam(3)CSK(4)-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-kappa B activation
Sang, Wei1; Zhong, Zhangfeng1,4; Linghu, Kegang1; Xiong, Wei1; Tse, Anfernee Kai Wing5; Cheang, Wai San1; Yu, Hua1,2,3,6; Wang, Yitao1,7
2018-07-05
Source PublicationCHINESE MEDICINE
ISSN1749-8546
Volume13
Abstract

Background

Siegesbeckia pubescens Makino (SP) is one of the important plant origins for the anti-inflammatory Chinese herbal medicine of Siegesbeckiae Herba. The current investigations indicated that the anti-inflammatory effects of SP were associated with the toll-like receptors (TLRs)-mediated nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) signaling pathways.

Methods

Raw 264.7 macrophages were pretreated with the 50% ethanol extract of SP (SPE, 50–200 µg/mL) and then co-treated with Pam3CSK4 (200 ng/mL) for another 12 h. The inhibitory effect of SPE on Pam3CSK4-stimulated NO release and post-inflammatory cytokines secretions were determined using Griess reagent and Elisa kits, respectively. The influence of SPE on NF-κB and MAPKs signaling relevant proteins was measured by Western blotting analysis, while the intracellular nitric oxide (NO) generation and NF-κB/p65 nuclear translocation were determined using Leica TCS SP8 laser scanning confocal microscope. Moreover, the effect of SPE on luciferase reporter gene in NF-κB-luc DNA transfected raw 264.7 cells was determined using the Dual-Glo luciferase assay system kit.

Results

SPE dose-dependently (50–200 µg/mL) attenuated Pam3CSK4-induced NO release, post-inflammatory cytokines (IL-6, TNF-α and MCP-1) secretions and intracellular NO generation in raw 264.7 cells. Biologically, SPE suppressed Pam3CSK4-induced expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), phosphorylation of NF-κB/p65 and IκBα, but did not significantly show effect on the proteins involved in MAPKs signaling (p38, ERK and JNK). The results were further confirmed by NF-κB-luc reporter gene assay and p65 nuclear translocation assay.

Conclusions

In conclusion, SPE ameliorated Pam3CSK4-induced inflammation in raw 264.7 cells through suppressing TLR 1/2-mediated NF-κB activation.

KeywordSiegesbeckia Pubescens Makino Pam(3)Csk(4) Inflammation Toll-like Receptor 1/2 Nf-kappa b
DOI10.1186/s13020-018-0193-x
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000437441000001
PublisherBMC
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorYu, Hua; Wang, Yitao
Affiliation1.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao, China.
2.HKBU Shenzhen Research Center, Shenzhen, Guangdong, China.
3.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
4.Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang, China
5.Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, Shenzhen, Guangdong, China
6.Institute of Chinese Medical Sciences, University of Macau, Room 8008, Building N22, Avenida da Universidade, Taipa, Macao SAR, China
7.Institute of Chinese Medical Sciences, University of Macau, Room 1050, Building N22, Avenida da Universidade, Taipa, Macao SAR, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Sang, Wei,Zhong, Zhangfeng,Linghu, Kegang,et al. Siegesbeckia pubescens Makino inhibits Pam(3)CSK(4)-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-kappa B activation[J]. CHINESE MEDICINE,2018,13.
APA Sang, Wei,Zhong, Zhangfeng,Linghu, Kegang,Xiong, Wei,Tse, Anfernee Kai Wing,Cheang, Wai San,Yu, Hua,&Wang, Yitao.(2018).Siegesbeckia pubescens Makino inhibits Pam(3)CSK(4)-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-kappa B activation.CHINESE MEDICINE,13.
MLA Sang, Wei,et al."Siegesbeckia pubescens Makino inhibits Pam(3)CSK(4)-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-kappa B activation".CHINESE MEDICINE 13(2018).
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