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Temperature Tolerance Electric Cell-Substrate Impedance Sensing for Joint Assessment of Cell Viability and Vitality
Yang, Ning1,2; Hui, Wenhao1; Dong, Sizhe2,3; Zhang, Xuming4; Shao, Liyang5; Jia, Yanwei2,3,6,7; Mak, Pui In2,3; Paulo Da Silva Martins, Rui2,3,8
2021-08-27
Source PublicationACS Sensors
ISSN2379-3694
Abstract

Evaluation of the cell health status is critical for drug screening and cell physiological activity investigations. The existing cell health assessment methods are solely devoted to the study of cell vitality or viability, leading to an incomplete evaluation. Herein, we report a convenient and robust method for the joint assessment of cell viability and vitality based on electric cell-substrate impedance sensing (ECIS) supplied with an environmental temperature control. The static value of electric cell-substrate impedance reflects the survival rate of cells, while the temperature tolerance of cells demonstrates the cell vitality. It was found that the cell vitality evaluated by the temperature tolerance of cells was independent of the initial cell numbers, rendering the proposed method easy to utilize in various applications. We compared the temperature tolerance ECIS method with the traditional trypan blue staining method, the methyl thiazolyl tetrazolium assay, and the direct impedance sensing method for joint evaluation of cell viability and vitality in drug screening. The temperature tolerance ECIS method showed comparable results but with a simpler protocol, faster results, and less dependence on the sample conditions. By providing both information on cell viability and cell vitality, the proposed temperature tolerance ECIS method would pave the way in building a simple and robust sensing system for cell health evaluation.

KeywordCell Viability Cell Vitality Drug Screening Electric Cell-substrate Impedance Sensing (Ecis) Temperature Tolerance
DOI10.1021/acssensors.1c01211
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Science & Technology - Other Topics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
Scopus ID2-s2.0-85114666913
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorJia, Yanwei
Affiliation1.School of Electrical and Information Engineering, Jiangsu University, Jiangsu, 212013, China
2.State-Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, University of Macau, 999078, Macao
3.Faculty of Science and Technology - ECE, University of Macau, 999078, Macao
4.Department of Applied Physics, Hong Kong Polytechnic University, Hong Kong, 999077, Hong Kong
5.Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen, 518071, China
6.Faculty of Health Sciences, University of Macau, 999078, Macao
7.MoE Frontiers Science Center for Precision Oncology, University of Macau, 999078, Macao
8.On Leave from Instituto Superior Técnico, Universidade de Lisboa, Lisboa, 1000-001, Portugal
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau;  Faculty of Science and Technology;  Faculty of Health Sciences
Recommended Citation
GB/T 7714
Yang, Ning,Hui, Wenhao,Dong, Sizhe,et al. Temperature Tolerance Electric Cell-Substrate Impedance Sensing for Joint Assessment of Cell Viability and Vitality[J]. ACS Sensors,2021.
APA Yang, Ning,Hui, Wenhao,Dong, Sizhe,Zhang, Xuming,Shao, Liyang,Jia, Yanwei,Mak, Pui In,&Paulo Da Silva Martins, Rui.(2021).Temperature Tolerance Electric Cell-Substrate Impedance Sensing for Joint Assessment of Cell Viability and Vitality.ACS Sensors.
MLA Yang, Ning,et al."Temperature Tolerance Electric Cell-Substrate Impedance Sensing for Joint Assessment of Cell Viability and Vitality".ACS Sensors (2021).
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