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Cell-based fluorescent microsphere incorporated with carbon dots as a sensitive immunosensor for the rapid detection of Escherichia coli O157 in milk
Yong Zhao1,2; Yanzhao Li2; Pingping Zhang1,2; Ziheng Yan1,2; Yugui Zhou1,2; Yipu Du1,2; Chunyu Qu2; Yajun Song1,2; Ding Zhou3; Songnan Qu4; Ruifu Yang1,2
2021-05-01
Source PublicationBiosensors and Bioelectronics
ISSN0956-5663
Volume179
Abstract

The rapid and early detection of foodborne pathogens in contaminated food is important for ensuring food safety and quality. In this study, a highly sensitive fluorescent immunosensor was developed to detect Escherichia coli O157:H7 in milk, by using microspheres labeled with carbon dots (CDs). The CDs-microspheres were prepared with Staphylococcus aureus cells as the carrier to incorporate CDs particles. Characterization of the microsphere revealed strong intensity, good stability and high uniformity in fluorescence. With Staphylococcal Protein A (SPA) on the surface of S. aureus cells, the microsphere could be easily coupled with various antibodies (e.g., immunoglobulin G). In combination with the immunomagnetic beads technique, a CDs-microsphere immunosensor was established for the specific detection of E. coli O157:H7 in milk. The limit of detection for E. coli O157:H7 is 2.4 × 10 colony-forming unit (CFU)/mL, comparable to that of real-time PCR methods. Milk samples spiked with E. coli O157:H7 at concentrations from 2.4 × 10 to 2.4 × 10 CFU/mL could be detected within 30 min. The coefficients of variation of the intra-assay tests were less than 10%, indicating a good repeatability. Moreover, the method was able to detect trace amounts of E. coli O157:H7 (<10 CFU) in real milk samples, with a 100% (10/10) accuracy after bacterial enrichment. This CDs-microsphere immunosensor shows considerable potential as a rapid and sensitive tool to detect pathogens in milk and other foods.

KeywordCarbon Dots Escherichia Coli O157:H7 Fluorescent Microspheres Staphylococcal Protein a Staphylococcus Aureus
DOI10.1016/j.bios.2021.113057
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000632848800003
Scopus ID2-s2.0-85100689433
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorDing Zhou; Songnan Qu; Ruifu Yang
Affiliation1.State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing,100071,China
2.Beijing Key Laboratory of POCT for Bioemergency and Clinic,Beijing,100071,China
3.State Key Laboratory of Luminescence and Applications,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Jilin,Changchun,130033,China
4.Joint Key Laboratory of the Ministry of Education,Institute of Applied Physics and Materials Engineering,University of Macau,Taipa,Macau,China
Corresponding Author AffilicationINSTITUTE OF APPLIED PHYSICS AND MATERIALS ENGINEERING
Recommended Citation
GB/T 7714
Yong Zhao,Yanzhao Li,Pingping Zhang,et al. Cell-based fluorescent microsphere incorporated with carbon dots as a sensitive immunosensor for the rapid detection of Escherichia coli O157 in milk[J]. Biosensors and Bioelectronics,2021,179.
APA Yong Zhao,Yanzhao Li,Pingping Zhang,Ziheng Yan,Yugui Zhou,Yipu Du,Chunyu Qu,Yajun Song,Ding Zhou,Songnan Qu,&Ruifu Yang.(2021).Cell-based fluorescent microsphere incorporated with carbon dots as a sensitive immunosensor for the rapid detection of Escherichia coli O157 in milk.Biosensors and Bioelectronics,179.
MLA Yong Zhao,et al."Cell-based fluorescent microsphere incorporated with carbon dots as a sensitive immunosensor for the rapid detection of Escherichia coli O157 in milk".Biosensors and Bioelectronics 179(2021).
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