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Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species
Ji,Shengle1; Gong,Qiyang2; Zhang,Wenwen3; Zheng,Jun3; Peng,Bo2; Yang,Menghua1
2020-12-01
Source PublicationFish and Shellfish Immunology
ISSN1050-4648
Volume107Pages:64-72
Abstract

Aquatic animals are frequently threated by bacterial pathogens. The most economic and efficient protection against bacterial infection are through vaccine immunization. The various serotypes of the pathogens, such as Vibrios, hurdle the development of the vaccines, especially polyvalent vaccines. Here, we demonstrate that recombinant bacterial ghost is a good candidate for multivalent vaccine. By expressing PhiX174 gene E alone or co-expressing the gene E with two genes encoding outer membrane proteins (VP1667 and VP2369) in V. parahaemolyticus, we generated the recombinant V. parahaemolyticus ghosts VPG and rVPGs respectively. Fish immunized with either VPG or rVPG showed increased survival against the infection by either V. parahaemolyticus or V. alginolyticus, with a better protective effect by immunization with rVPG. Our furthermore studies show that rVPG stimulates stronger innate immune responses by increasing the expression of tnfα, il1β, il6, il8 and il10 as well as that of c3b, lyz, and tlr5, the key players linking the innate and adaptive immune responses upon microbial stimulation. In summary, VPG and rVPG can protect zebrafish against the infection from at least two Vibrio species, suggesting its potential value for further aquaculture vaccines development.

KeywordBacterial Ghosts Immunoprotection Polyvalent Vaccine Vibrio Parahaemolyticus
DOI10.1016/j.fsi.2020.10.009
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000599882200008
Scopus ID2-s2.0-85092475458
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorPeng,Bo; Yang,Menghua
Affiliation1.College of Animal Science and Technology,College of Veterinary Medicine,Zhejiang A & F University,Hangzhou,311300,China
2.State Key Laboratory of Biocontrol,Guangdong Key Laboratory of Pharmaceutical Functional Genes,School of Life Sciences,Sun Yat-sen University,Guangzhou,510275,China
3.Faculty of Health Sciences,University of Macau,Macau SAR,China
Recommended Citation
GB/T 7714
Ji,Shengle,Gong,Qiyang,Zhang,Wenwen,et al. Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species[J]. Fish and Shellfish Immunology,2020,107:64-72.
APA Ji,Shengle,Gong,Qiyang,Zhang,Wenwen,Zheng,Jun,Peng,Bo,&Yang,Menghua.(2020).Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species.Fish and Shellfish Immunology,107,64-72.
MLA Ji,Shengle,et al."Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species".Fish and Shellfish Immunology 107(2020):64-72.
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