UM  > Faculty of Health Sciences
Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging
Liu,Zhihe1,2; Liu,Jie1,3; Zhang,Zhe2; Sun,Zezhou2; Shao,Xuyang1; Guo,Jintong1; Xi,Lei1; Yuan,Zhen4; Zhang,Xuanjun4; Chiu,Daniel T.5; Wu,Changfeng1
2020-04-14
Source PublicationNanoscale
ISSN2040-3364
Volume12Issue:14Pages:7522-7526
Abstract

Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

DOI10.1039/d0nr00347f
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS IDWOS:000529531500008
Fulltext Access
Citation statistics
Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorWu,Changfeng
Affiliation1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen Guangdong,510855,China
2.State Key Laboratory of Integrated Optoelectronics,College of Electronic Science and Engineering,Jilin University,Changchun Jilin,130012,China
3.Department of Biology,Hong Kong Baptist University,Hong Kong,Hong Kong
4.Faculty of Health Science,University of Macau,Taipa,999078,Macao
5.Department of Chemistry,University of Washington,Seattle,98195,United States
Recommended Citation
GB/T 7714
Liu,Zhihe,Liu,Jie,Zhang,Zhe,et al. Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging[J]. Nanoscale,2020,12(14):7522-7526.
APA Liu,Zhihe.,Liu,Jie.,Zhang,Zhe.,Sun,Zezhou.,Shao,Xuyang.,...&Wu,Changfeng.(2020).Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging.Nanoscale,12(14),7522-7526.
MLA Liu,Zhihe,et al."Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging".Nanoscale 12.14(2020):7522-7526.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu,Zhihe]'s Articles
[Liu,Jie]'s Articles
[Zhang,Zhe]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu,Zhihe]'s Articles
[Liu,Jie]'s Articles
[Zhang,Zhe]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu,Zhihe]'s Articles
[Liu,Jie]'s Articles
[Zhang,Zhe]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.