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Sphingolipidomic analysis of c. Elegans reveals development-and environment-dependent metabolic features
Cheng,Xiaoxiang1; Jiang,Xue1; Tam,Kin Yip1; Li,Gang1; Zheng,Jun1; Zhang,Hongjie1,2
2019
Source PublicationInternational Journal of Biological Sciences
ISSN1449-2288
Volume15Issue:13Pages:2897-2910
Abstract

Sphingolipids (SLs) serve as structural and signaling molecules in regulating various cellular events and growth. Given that SLs contain various bioactive species possessing distinct roles, quantitative analysis of sphingolipidome is essential for elucidating their differential requirement during development. Herein we developed a comprehensive sphingolipidomic profiling approach using liquid chromatography-mass spectrometry coupled with multiple reaction monitoring mode (LC-MS-MRM). SL profiling of C. elegans revealed organism-specific, development-dependent and environment-driven metabolic features. We showed for the first time the presence of a series of sphingoid bases in C. elegans sphingolipid profiles, although only C17-sphingoid base is used for generating complex SLs. Moreover, we successfully resolved growth-, temperature-and nutrition-dependent SL profiles at both individual metabolite-level and network-level. Sphingolipidomic analysis uncovered significant SL composition changes throughout development, with SMs/GluCers ratios dramatically increasing from larva to adult stage whereas total sphingolipid levels exhibiting opposing trends. We also identified a temperature-dependent alteration in SMs/GluCers ratios, suggesting an organism-specific strategy for environmental adaptation. Finally, we found serine-biased GluCer increases between serine-versus alanine-supplemented worms. Our study builds a “reference” resource for future SL analysis in the worm, provides insights into natural variability and plasticity of eukaryotic multicellular sphingolipid composition and is highly valuable for investigating their functional significance.

KeywordCaenorhabditis Elegans Development Lc/ms/mrm Nutrition Sphingolipidomics Temperature
DOI10.7150/ijbs.30499
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000496826300011
Scopus ID2-s2.0-85075666161
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorZhang,Hongjie
Affiliation1.Faculty of Health Sciences,University of Macau,Taipa,999078,Macao
2.Centre of Reproduction,Development and Ageing,University of Macau,Taipa,999078,Macao
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences;  University of Macau
Recommended Citation
GB/T 7714
Cheng,Xiaoxiang,Jiang,Xue,Tam,Kin Yip,et al. Sphingolipidomic analysis of c. Elegans reveals development-and environment-dependent metabolic features[J]. International Journal of Biological Sciences,2019,15(13):2897-2910.
APA Cheng,Xiaoxiang,Jiang,Xue,Tam,Kin Yip,Li,Gang,Zheng,Jun,&Zhang,Hongjie.(2019).Sphingolipidomic analysis of c. Elegans reveals development-and environment-dependent metabolic features.International Journal of Biological Sciences,15(13),2897-2910.
MLA Cheng,Xiaoxiang,et al."Sphingolipidomic analysis of c. Elegans reveals development-and environment-dependent metabolic features".International Journal of Biological Sciences 15.13(2019):2897-2910.
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