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A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction
Xie,Shuping1; Li,Bin1; Lyu,Peng2; Kwok,Hang Fai3; Ge,Lilin1; Wu,Qinan1
2020-03-14
Source PublicationNew Journal of Chemistry
ISSN1144-0546
Volume44Issue:10Pages:3820-3823
AbstractA new electrochemical immunoassay was designed for the sensitive detection of prostate-specific antigen (PSA) via a pyrophosphatase (PPase)-hydrolysed Cu(ii)-coordinated pyrophosphate ion (Cu-PPi) complex, accompanying the release of Cu(ii) ions and capture on a negatively charged electrode. This system involved a sandwiched immunoreaction with nanogold-labeled PPase/detection antibodies on a polystyrene microplate, enzymatic hydrolysis, Cu(ii) release and voltammetric measurement. The voltammetric signal is derived from the as-released Cu(ii) ions within the applied potential on the electrode. Under optimum conditions, our strategy exhibited a good voltammetric response for the analyte, and allowed determination of PSA at a concentration as low as 5.2 pg mL. Good precision, high specificity and acceptable accuracy were achieved for the detection of PSA.
DOI10.1039/c9nj05514b
URLView the original
Language英语
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing,210000,China
2.College of Biological Science and Technology,Fuzhou University,Fuzhou Fujian,350108,China
3.Cancer Centre,Faculty of Health Sciences,University of Macau,Taipa,Avenida de Universidade,Macao
Recommended Citation
GB/T 7714
Xie,Shuping,Li,Bin,Lyu,Peng,et al. A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction[J]. New Journal of Chemistry,2020,44(10):3820-3823.
APA Xie,Shuping,Li,Bin,Lyu,Peng,Kwok,Hang Fai,Ge,Lilin,&Wu,Qinan.(2020).A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction.New Journal of Chemistry,44(10),3820-3823.
MLA Xie,Shuping,et al."A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction".New Journal of Chemistry 44.10(2020):3820-3823.
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