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Pharmacological effects of a novel bradykinin-related peptide (RR-18) from the skin secretion of the hejiang frog (Ordorrana hejiangensis) on smooth muscle
Zhou,Xiaowei1,2; Xu,Jie2; Zhong,Ruimin3; Ma,Chengbang2; Zhou,Mei2; Cao,Zhijian4; Xi,Xinping2; Shaw,Chris2; Chen,Tianbao2; Wang,Lei2; Kwok,Hang Fai1
2020-07-01
Source PublicationBiomedicines
Volume8Issue:7
AbstractBradykinin (BK) and bradykinin-related peptides (BRPs), which were identified from a diversity of amphibian skin secretions, exerted contractile and relaxing effects on non-vascular and vascular smooth muscle, respectively. Here, we report a novel bradykinin-related peptide with a molecular mass of 1890.2 Da, RVAGPDKPARISGLSPLR, which was isolated and identified from Ordorrana hejiangensis skin secretions, followed by a C-terminal extension sequence VAPQIV. The biosynthetic precursor-encoding cDNA was cloned by the "shotgun" cloning method, and the novel RR-18 was identified and structurally confirmed by high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS). Subsequently, the myotropic activity of the synthetic replicate of RR-18 was investigated on the rat bladder, uterus, tail artery and ileum smooth muscle. The peptide was named RR-18 in accordance (R = N-terminal arginine, R = C-terminal arginine, 18 = number of residues). In this study, the synthetic replicates of RR-18 showed no agonist/antagonism of BK-induced rat bladder and uterus smooth muscle contraction. However, it displayed an antagonism of bradykinin-induced rat ileum contraction and arterial smooth muscle relaxation. The EC values of BK for ileum and artery, were 214.7 nM and 18.3 nM, respectively. When the tissue was pretreated with the novel peptide, RR-18, at the maximally effective concentration of bradykinin (1 × 10 M), bradykinin-induced contraction of the ileum and relaxation of the arterial smooth muscle was reduced by 50-60% and 30-40%, respectively. In conclusion, RR-18 represents novel bradykinin antagonising peptide from amphibian skin secretions. It may provide new insight into possible treatment options for chronic pain and chronic inflammation.
KeywordAntagonist Bradykinin related peptide Frog Skin secretion Smooth muscle
DOI10.3390/BIOMEDICINES8070225
URLView the original
Language英語English
Scopus ID2-s2.0-85089683980
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorXi,Xinping
Affiliation1.Institute of Translational Medicine,University of Macau,Taipa, Macau,Avenida da Universidade,China
2.Natural Drug Discovery Group,School of Pharmacy,Queen's University,Belfast,BT9 7BL,United Kingdom
3.Department of Nutrition,Henry Fok School of Food Science and Engineering,Shaoguan University,Shaoguan,512005,China
4.State Key Laboratory of Virology,Modern Virology Research Center,College of Life Sciences,Wuhan University,Wuhan,430072,China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Zhou,Xiaowei,Xu,Jie,Zhong,Ruimin,et al. Pharmacological effects of a novel bradykinin-related peptide (RR-18) from the skin secretion of the hejiang frog (Ordorrana hejiangensis) on smooth muscle[J]. Biomedicines,2020,8(7).
APA Zhou,Xiaowei,Xu,Jie,Zhong,Ruimin,Ma,Chengbang,Zhou,Mei,Cao,Zhijian,Xi,Xinping,Shaw,Chris,Chen,Tianbao,Wang,Lei,&Kwok,Hang Fai.(2020).Pharmacological effects of a novel bradykinin-related peptide (RR-18) from the skin secretion of the hejiang frog (Ordorrana hejiangensis) on smooth muscle.Biomedicines,8(7).
MLA Zhou,Xiaowei,et al."Pharmacological effects of a novel bradykinin-related peptide (RR-18) from the skin secretion of the hejiang frog (Ordorrana hejiangensis) on smooth muscle".Biomedicines 8.7(2020).
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