UM
Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from the Skin Secretion of Odorrana schmackeri
Zhou,Xiaowei1,2,3; Liu,Yue3; Gao,Yitian4; Wang,Yuanxing5; Xia,Qiang6; Zhong,Ruimin1; Ma,Chengbang3; Zhou,Mei3; Xi,Xinping3; Shaw,Chris3; Chen,Tianbao3; Wu,Di7; Kwok,Hang Fai2; Wang,Lei3
2020-08-01
Source PublicationToxins
Volume12Issue:8
AbstractAntimicrobial peptides (AMPs) are promising therapeutic alternatives compared to conventional antibiotics for the treatment of drug-resistant bacterial infections. However, the application of the overwhelming majority of AMPs is limited because of the high toxicity and high manufacturing costs. Amphibian skin secretion has been proven to be a promising source for the discovery and development of novel AMPs. Herein, we discovered a novel AMP from the skin secretion of Odorrana schmackeri, and designed the analogues by altering the key factors, including conformation, net charge and amphipathicity, to generate short AMPs with enhanced therapeutic efficacy. All the peptides were chemically synthesised, followed by evaluating their biological activity, stability and cytotoxicity. OSd, OSe and OSf exhibited broad-spectrum antibacterial effects, especially OSf, which presented the highest therapeutic index for the tested bacteria. Moreover, these peptides displayed good stability. The results from scanning electron microscopy and transmission electron microscopy studies, indicated that brevinin-OS, OSd, OSe and OSf possessed rapid bactericidal ability by disturbing membrane permeability and causing the release of cytoplasmic contents. In addition, OSd, OSe and OSf dramatically decreased the mortality of waxworms acutely infected with MRSA. Taken together, these data suggested that a balance between positive charge, degrees of α-helicity and hydrophobicity, is necessary for maintaining antimicrobial activity, and these data successfully contributed to the design of short AMPs with significant bactericidal activity and cell selectivity.
KeywordAntimicrobial activity Skin-derived peptides Stability Waxworm model
DOI10.3390/toxins12080484
URLView the original
Language英语
Fulltext Access
Citation statistics
Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorXi,Xinping
Affiliation1.Department of Nutrition,Henry Fok School of Food Science and Engineering,Shaoguan University,Shaoguan,512005,China
2.Institute of Translational Medicine,Faculty of Health Sciences,University of Macau,Taipa,Avenida da Universidade,Macao
3.Natural Drug Discovery Group,School of Pharmacy,Queen's University,Belfast, Northern Ireland,BT9 7BL,United Kingdom
4.College of Life and Environmental Science,Wenzhou University,Wenzhou,325035,China
5.State Key Lab of Food Science and Technology,Nanchang University,Nanchang,330047,China
6.Department of Food Science and Engineering,Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province,College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,315832,China
7.Chemical Biology Research Centre,School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,325035,China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zhou,Xiaowei,Liu,Yue,Gao,Yitian,et al. Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from the Skin Secretion of Odorrana schmackeri[J]. Toxins,2020,12(8).
APA Zhou,Xiaowei.,Liu,Yue.,Gao,Yitian.,Wang,Yuanxing.,Xia,Qiang.,...&Wang,Lei.(2020).Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from the Skin Secretion of Odorrana schmackeri.Toxins,12(8).
MLA Zhou,Xiaowei,et al."Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from the Skin Secretion of Odorrana schmackeri".Toxins 12.8(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou,Xiaowei]'s Articles
[Liu,Yue]'s Articles
[Gao,Yitian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou,Xiaowei]'s Articles
[Liu,Yue]'s Articles
[Gao,Yitian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou,Xiaowei]'s Articles
[Liu,Yue]'s Articles
[Gao,Yitian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.