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Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes
Li,Bin1,2; Ge,Lilin1; Lyu,Peng3; Chen,Meijuan4; Zhang,Xiongfei4; Xie,Shuping1,5; Wu,Qinan1; Kwok,Hang Fai2
2021
Source PublicationMicrochimica Acta
ISSN0026-3672
Volume188Issue:1
AbstractA simple and feasible pH meter–based immunoassay is reported for detection of C-reactive protein (CRP) using glucose oxidase (GOD)–conjugated dendrimer loaded with platinum nanozyme. Initially, platinum nanozymes were loaded into the dendrimers through an in situ synthetic method. Then, GOD and monoclonal anti-CRP antibody with a high molar ratio were covalently conjugated onto carboxylated dendrimers via typical carbodiimide coupling. The immunoreaction was carried out with a competitive mode in a CRP-coated microplate. Along with formation of immunocomplex, the added glucose was oxidized into gluconic acid and hydrogen peroxide by GOD, and the latter was further decomposed by platinum nanozyme, thus accelerating chemical reaction in the positive direction. The produced gluconic acid changed the pH of detection solution, which was determined using a handheld pH meter. Under optimum conditions, the pH meter–based immunoassay gave a good signal toward target CRP from 0.01 to 100 ng mL. The limit of detection was 5.9 pg mL. An intermediate precision ≤ 11.2% was acquired with batch-to-batch identification. No nonspecific adsorption was observed during a series of procedures to detect target CRP, and the cross-reaction against other biomarkers was very low. Importantly, our system gave well-matched results for analysis of human serum samples relative to a referenced ELISA kit. Graphical abstract[Figure not available: see fulltext.].
KeywordC-reactive protein Enzymatic cascade amplification Nanoparticle-encapsulated dendrimer pH detection Platinum nanozyme Potentiometric immunoassay
DOI10.1007/s00604-020-04687-9
URLView the original
Language英语
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorGe,Lilin
Affiliation1.Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing,210023,China
2.Institute of Translational Medicine,Faculty of Health Sciences,University of Macau,Taipa,Avenida de Universidade,Macao
3.College of Biological Science and Technology,Fuzhou University,Fuzhou,350108,China
4.School of Medicine & Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China
5.Division of Life Science,Hong Kong University of Science and Technology,Hong Kong
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Li,Bin,Ge,Lilin,Lyu,Peng,et al. Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes[J]. Microchimica Acta,2021,188(1).
APA Li,Bin.,Ge,Lilin.,Lyu,Peng.,Chen,Meijuan.,Zhang,Xiongfei.,...&Kwok,Hang Fai.(2021).Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes.Microchimica Acta,188(1).
MLA Li,Bin,et al."Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes".Microchimica Acta 188.1(2021).
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