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Boronate ester bond–based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode
Chen,Ye1,2; Li,Bin2,3; Lyu,Peng4; Kwok,Hang Fai3; Ge,Lilin1,2; Wu,Qinan2
2021-02-01
Source PublicationAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume413Issue:4Pages:1073-1080
AbstractPhenylboronic acid-functionalized nanometer-sized CaCO particles (PBA-CaCO) were designed to determine the carcinoembryonic antigen (CEA) glycoprotein with a portable Ca ion-selective electrode (Ca-ISE) through a typical boronate ester bond. CaCO nanospheres were conjugated to 3-aminophenylboronic acid by amine-epoxy reaction, whereas target CEA was captured into the aptasensing interface by the immobilized thiolated aptamer on gold substrate. Upon PBA-CaCO introduction, 3-aminophenylboronic acid labeled to CaCO microsphere specifically recognized with CEA glycoprotein based on sugar-boronic acid interaction to form a sandwiched complex. The carried CaCO was dissolved under acidic conditions to release Ca ion with a portable Ca-ISE readout. Thanks to the specific boronate ester bond between PBA and 1,2-diols, the synthesized PBA-CaCO exhibited good conjugation properties for CEA glycoprotein. Under optimum conditions, Ca-ISE-based aptasensing platform exhibited good electrode potential response for evaluation of target CEA, and allowed detection of CEA at a concentration as low as 7.3 pg mL. Importantly, Ca-ISE-based aptasensing system is readily extended to detect other disease-related glycoproteins by controlling the corresponding aptamer.
KeywordAptasensor Carcinoembryonic antigen Ion-selective electrode Nanometer-sized CaCO3 Potentiometric measurement
DOI10.1007/s00216-020-03067-9
URLView the original
Language英語English
Scopus ID2-s2.0-85096455248
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorChen,Ye
Affiliation1.Changzhou Fourth People’s Hospital,Changzhou,213000,China
2.Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing,210023,China
3.Cancer Centre,Faculty of Health Sciences,University of Macau,Taipa,Avenida da Universidade,Macao
4.College of Biological Science and Technology,Fuzhou University,Fuzhou,350108,China
Recommended Citation
GB/T 7714
Chen,Ye,Li,Bin,Lyu,Peng,et al. Boronate ester bond–based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode[J]. Analytical and Bioanalytical Chemistry,2021,413(4):1073-1080.
APA Chen,Ye,Li,Bin,Lyu,Peng,Kwok,Hang Fai,Ge,Lilin,&Wu,Qinan.(2021).Boronate ester bond–based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode.Analytical and Bioanalytical Chemistry,413(4),1073-1080.
MLA Chen,Ye,et al."Boronate ester bond–based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode".Analytical and Bioanalytical Chemistry 413.4(2021):1073-1080.
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