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Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence
Zhang W.1; Xie R.1; Zhang X.D.1; Lee L.T.O.1; Zhang H.1; Yang M.2; Peng B.3,4; Zheng J.1,5
2020
Source PublicationFASEB Journal
ISSN0892-6638
Volume34Issue:6Pages:7561-7577
Abstract

Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the host of pathogen has emerged as a novel strategy to understand the responses concurrently from both pathogen and host at cellular level. However, bacterial infection mostly causes systematic responses from the host at organism level where the interplay is urgently to be understood but inevitably being neglected by the current practice. Here, we developed an approach that simultaneously monitor the genome-wide infection-linked transcriptional alterations in both pathogenic Vibrio parahaemolyticus and the infection host nematode Caenorhabditis elegans. Besides the dynamic alterations in transcriptomes of both C. elegans and V. parahaemolyticus during infection, we identify a two-component system, BarA/UvrY, that is important for virulence in host. BarA/UvrY not only controls the virulence factors in V. parahaemolyticus including Type III and Type VI secretion systems, but also attenuates innate immune responses in C. elegans, including repression on the MAP kinase-mediated cascades. Thus, our study exemplifies the use of dual RNA-Seq at organism level to uncover previously unrecognized interplay between host and pathogen.

KeywordBara C. Elegans Organism Dual Rna-seq Uvry V. Parahaemolyticus Virulence Factor
DOI10.1096/fj.201902630R
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biology ; Cell Biology
WOS IDWOS:000526117700001
The Source to ArticleScopus
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Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorZheng J.
Affiliation1.Faculty of Health Sciences, University of Macau, Macau;
2.Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, Zhejiang A&F University, Hangzhou, China;
3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China;
4.School of Life Sciences, Sun Yat-sen University, Guangzhou, China;
5.Institute of Translational Medicine, University of Macau, Macau
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences;  University of Macau
Recommended Citation
GB/T 7714
Zhang W.,Xie R.,Zhang X.D.,et al. Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence[J]. FASEB Journal,2020,34(6):7561-7577.
APA Zhang W..,Xie R..,Zhang X.D..,Lee L.T.O..,Zhang H..,...&Zheng J..(2020).Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence.FASEB Journal,34(6),7561-7577.
MLA Zhang W.,et al."Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence".FASEB Journal 34.6(2020):7561-7577.
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