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Disruption of dmrt1 rescues the all-male phenotype of the cyp19a1a mutant in zebrafish - A novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis
Wu,Kun; Song,Weiyi; Zhang,Zhiwei; Ge,Wei
2020
Source PublicationDevelopment (Cambridge)
ISSN0950-1991
Volume147Issue:4
AbstractSex determination and differentiation are complex processes controlled by many different factors; however, the relationships among these factors are poorly understood. Zebrafish gonadal differentiation exhibits high plasticity involving multiple factors and pathways, which provides an excellent model for investigating the interactions between them. Ovarian aromatase (cyp19a1a) and dmrt1 are key factors in directing vertebrate ovary and testis differentiation, respectively. Knockout of zebrafish cyp19a1a leads to all-male offspring, whereas the loss of dmrt1 results in a femalebiased sex ratio. In the present study, we established dmrt1; cyp19a1a double mutant zebrafish and discovered that the introduction of the dmrt1 mutation into the cyp19a1a mutant could rescue the all-male phenotype of the latter. Interestingly, despite the lack of aromatase/estrogens, the follicles in the ovary of the rescued cyp19a1a mutant could develop normally up to the previtellogenic stage. Further evidence suggested the ovarian aromatase directed ovarian differentiation by suppressing dmrt1 expression via nuclear estrogen receptors (nERs). Our results provide solid evidence for an interaction between cyp19a1a and dmrt1 in zebrafish gonadal differentiation, and for the dispensability of estrogens in controlling early folliculogenesis.
KeywordAromatase Cyp19a1a Dmrt1 Folliculogenesis Sex differentiation Zebrafish
DOI10.1242/dev.182758
URLView the original
Language英语
Scopus ID2-s2.0-85080986370
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorGe,Wei
AffiliationCentre of Reproduction Development and Aging (CRDA),Faculty of Health Sciences,University of Macau,Taipa,999078,Macao
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Wu,Kun,Song,Weiyi,Zhang,Zhiwei,et al. Disruption of dmrt1 rescues the all-male phenotype of the cyp19a1a mutant in zebrafish - A novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis[J]. Development (Cambridge),2020,147(4).
APA Wu,Kun,Song,Weiyi,Zhang,Zhiwei,&Ge,Wei.(2020).Disruption of dmrt1 rescues the all-male phenotype of the cyp19a1a mutant in zebrafish - A novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis.Development (Cambridge),147(4).
MLA Wu,Kun,et al."Disruption of dmrt1 rescues the all-male phenotype of the cyp19a1a mutant in zebrafish - A novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis".Development (Cambridge) 147.4(2020).
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