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Genetic evidence for estrogenicity of bisphenol A in zebrafish gonadal differentiation and its signalling mechanism
Song,Weiyi1; Lu,Huijie1; Wu,Kun1; Zhang,Zhiwei1; Shuk-Wa Lau,Esther2; Ge,Wei1
2020-03-15
Source PublicationJournal of Hazardous Materials
ISSN0304-3894
Volume386
AbstractBisphenol A (BPA) can induce endocrine disorders in humans and animals. In this study, we used several zebrafish mutants deficient in estrogen production and signalling, which could be valuable for evaluating estrogenic activities and mechanisms of EDCs. With low endogenous estrogens, the all-male aromatase mutant (cyp19a1a) is expected to be more responsive to estrogenic exposure, and mutants of nuclear estrogen receptors (nERs; esr1, esr2a and esr2b) alone or in combination would allow us to evaluate the action mechanisms of estrogenic EDCs. Exposure to BPA could rescue the all-male phenotype of the cyp19a1a mutant, delayed gonadal development in both sexes, resulting in infertility or subfertility, and caused follicle atresia in females and impairment of spermatogenesis in males. To understand the mechanisms of these effects, we tested BPA in cyp19a1a and nER mutants of different combinations. The feminizing effect of BPA on sexual differentiation was dependent on nERs, in particular esr2a. As for males, nERs were also involved in BPA-induced impairment of spermatogenesis. Taken together, with genome editing technology our study provides the most comprehensive genetic evidence for estrogenic activities of BPA in zebrafish and its action mechanisms. This study also establishes a powerful platform for studying other EDCs with estrogenic activity.
KeywordAromatase Bisphenol A Estrogen receptors Genome editing Gonadal differentiation Zebrafish
DOI10.1016/j.jhazmat.2019.121886
URLView the original
Language英语
Scopus ID2-s2.0-85076899585
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorGe,Wei
Affiliation1.Centre of Reproduction,Development and Aging (CRDA),Faculty of Health Sciences,University of Macau,Taipa,999078,China
2.School of Life Sciences,The Chinese University of Hong Kong,Shatin,New Territories,999077,Hong Kong
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Song,Weiyi,Lu,Huijie,Wu,Kun,et al. Genetic evidence for estrogenicity of bisphenol A in zebrafish gonadal differentiation and its signalling mechanism[J]. Journal of Hazardous Materials,2020,386.
APA Song,Weiyi,Lu,Huijie,Wu,Kun,Zhang,Zhiwei,Shuk-Wa Lau,Esther,&Ge,Wei.(2020).Genetic evidence for estrogenicity of bisphenol A in zebrafish gonadal differentiation and its signalling mechanism.Journal of Hazardous Materials,386.
MLA Song,Weiyi,et al."Genetic evidence for estrogenicity of bisphenol A in zebrafish gonadal differentiation and its signalling mechanism".Journal of Hazardous Materials 386(2020).
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