UM  > Faculty of Health Sciences
Genome and transcriptome sequencing of casper and roy zebrafish mutants provides novel genetic clues for iridophore loss
Bian,Chao1,2; Chen,Weiting1,3; Ruan,Zhiqiang2,4; Hu,Zhe1; Huang,Yu2,4; Lv,Yunyun2,5; Xu,Tengfei2; Li,Jia2; Shi,Qiong2,4; Ge,Wei1
2020-04-01
Source PublicationInternational Journal of Molecular Sciences
ISSN1661-6596
Volume21Issue:7
Abstractcasper has been a widely used transparent mutant of zebrafish. It possesses a combined loss of reflective iridophores and light-absorbing melanophores, which gives rise to its almost transparent trunk throughout larval and adult stages. Nevertheless, genomic causal mutations of this transparent phenotype are poorly defined. To identify the potential genetic basis of this fascinating morphological phenotype, we constructed genome maps by performing genome sequencing of 28 zebrafish individuals including wild-type AB strain, roy orbison (roy), and casper mutants. A total of 4.3 million high-quality and high-confidence homozygous single nucleotide polymorphisms (SNPs) were detected in the present study. We also identified a 6.0-Mb linkage disequilibrium block specifically in both roy and casper that was composed of 39 functional genes, of which the mpv17 gene was potentially involved in the regulation of iridophore formation and maintenance. This is the first report of high-confidence genomic mutations in the mpv17 gene of roy and casper that potentially leads to defective splicing as one major molecular clue for the iridophore loss. Additionally, comparative transcriptomic analyses of skin tissues from the AB, roy and casper groups revealed detailed transcriptional changes of several core genes that may be involved in melanophore and iridophore degeneration. In summary, our updated genome and transcriptome sequencing of the casper and roy mutants provides novel genetic clues for the iridophore loss. These new genomic variation maps will offer a solid genetic basis for expanding the zebrafish mutant database and in-depth investigation into pigmentation of animals.
KeywordCasper Genome sequencing Iridophore degeneration Roy Transcriptome sequencing Variation
DOI10.3390/ijms21072385
URLView the original
Language英语
Fulltext Access
Citation statistics
Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorShi,Qiong
Affiliation1.Centre of Reproduction,Development and Aging,Faculty of Health Sciences,University of Macau,Taipa,999078,China
2.Shenzhen Key Lab of Marine Genomics,Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals,BGI Academy of Marine Sciences,BGI Marine,BGI,Shenzhen,518083,China
3.School of Life Sciences,Jiaying University,Meizhou,514015,China
4.BGI Education Center,University of Chinese Academy of Sciences,Shenzhen,518083,China
5.College of Life Sciences,Neijiang Normal University,Neijiang,641100,China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Bian,Chao,Chen,Weiting,Ruan,Zhiqiang,et al. Genome and transcriptome sequencing of casper and roy zebrafish mutants provides novel genetic clues for iridophore loss[J]. International Journal of Molecular Sciences,2020,21(7).
APA Bian,Chao.,Chen,Weiting.,Ruan,Zhiqiang.,Hu,Zhe.,Huang,Yu.,...&Ge,Wei.(2020).Genome and transcriptome sequencing of casper and roy zebrafish mutants provides novel genetic clues for iridophore loss.International Journal of Molecular Sciences,21(7).
MLA Bian,Chao,et al."Genome and transcriptome sequencing of casper and roy zebrafish mutants provides novel genetic clues for iridophore loss".International Journal of Molecular Sciences 21.7(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Bian,Chao]'s Articles
[Chen,Weiting]'s Articles
[Ruan,Zhiqiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Bian,Chao]'s Articles
[Chen,Weiting]'s Articles
[Ruan,Zhiqiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Bian,Chao]'s Articles
[Chen,Weiting]'s Articles
[Ruan,Zhiqiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.