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Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation
Ma,Qiu Shuo1; Linghu,Ke Gang1; Zhang,Tian1; Zhao,Guan Ding1; Xiong,Wei1; Xiong,Shi Hang1; Zhao,Mingming1; Xu,Wei2; Yu,Juan3; Yu,Hua1,2,4
2020-08-27
Source PublicationChinese Medicine (United Kingdom)
Volume15Issue:1
Abstract

Background: Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extract (SGE) on collagen-induced arthritic rats and interleukin (IL)-1β-stimulated human synovial SW982 cells. Methods: Rats were induced to arthritis by collagen for 15 days and then received SGE treatment for 35 days. The body weight and arthritis severity score of the rats were monitored weekly. At the end of the experiment, the radiographic and histological changes of rats' hind paw were obtained; the serum C-reactive protein was detected by enzyme-linked immunosorbent assay (ELISA); the expression levels of interleukin (IL)- 1β, IL6 and IL-10 in the joint muscles were determined by ELISA and immunohistochemical staining; and the level of regulatory T cells (Tregs) in the spleen was detected using flow cytometry. In addition, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were used to evaluate the proliferation of SW982 synovial cells. ELISA, western blot and immunofluorescence staining were used to investigate the anti-inflammatory mechanisms of SGE on IL-1β-induced SW982 cells and joint muscles of CIA rats. Results: SGE attenuated the collagen-induced hind paw swelling, cartilage damage and bone erosion. SGE inhibited the synovial hyperplasia to the articular cavity in the toe joint and ankle. Moreover, SGE decreased the production of C-reactive protein in serum and the expression of IL-6, IL-1β, cyclooxygenase-2 (COX-2) and phosphorylation of NF-κB p65 in the joint muscles. SGE also recovered the decreased Tregs. Results from the in vitro experiments showed that SGE not only inhibited the proliferation and migration of human synovial cell but also inhibited the IL-1β-induced expression of IL-6 and IL-8. Similarly, SGE inhibited the activation of NF-κB and the expression of COX-2. Conclusions: SGE attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation.[Figure not available: see fulltext.]

KeywordInflammation Nuclear Transcription Factor-kappa b Rheumnnatoid Arthritis Sigesbeckia Glabrescens Makino Sw982 Synovial Hyperplasia
DOI10.1186/s13020-020-00372-4
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000565975100001
Scopus ID2-s2.0-85092034972
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYu,Hua
Affiliation1.Institute of Chinese Medical Sciences,State Key Laboratory of Quality Research in Chinese Medicine,University of Macau,Taipa, Macao,Avenida da Universidade,Macao
2.College of Pharmacy,Fujian University,Traditional Chinese Medicine,Fuzhou, Fujian,China
3.Zhangzhou Pientzehuang Pharmaceutical Ltd,Zhangzhou, Fujian,China
4.HKBU Shenzhen Research Center,Shenzhen, Guangdong,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ma,Qiu Shuo,Linghu,Ke Gang,Zhang,Tian,et al. Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation[J]. Chinese Medicine (United Kingdom),2020,15(1).
APA Ma,Qiu Shuo,Linghu,Ke Gang,Zhang,Tian,Zhao,Guan Ding,Xiong,Wei,Xiong,Shi Hang,Zhao,Mingming,Xu,Wei,Yu,Juan,&Yu,Hua.(2020).Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation.Chinese Medicine (United Kingdom),15(1).
MLA Ma,Qiu Shuo,et al."Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation".Chinese Medicine (United Kingdom) 15.1(2020).
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