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Adsorbed hollow fiber immobilized tyrosinase for the screening of enzyme inhibitors from Pueraria lobata extract
Zhao,Cong Peng1; Yin,Shi Jun1; Chen,Guo Ying1; Wang,Yuan1; Chen,Hua1; Zhao,Jing2; Yang,Feng Qing1
2021-01-30
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN0731-7085
Volume193
AbstractIn this study, a method based on adsorbed hollow fiber immobilized tyrosinase (TYR) was developed to screening potential TYR inhibitors from Pueraria lobate extract. Kojic acid and ranitidine were used as positive and negative control to verify the reliability of the proposed method, respectively. Several significant parameters of the screening process, including the amount of P. lobata extract, adsorption time and incubation time, were optimized. After investigating the repeatability of the developed method, seven potential active compounds in P. lobata extract were successfully detected and their chemical structures were tentatively identified by liquid chromatography – mass spectrometry analysis. Furthermore, the inhibitory activity of four identified compounds on TYR was tested in vitro, and three of them, namely, puerarin, puerarin-6″-O-xyloside and puerarin apioside were verified to have good TYR inhibitory activity with IC value of 478.5, 513.8, and 877.3 μM, respectively. In addition, the molecular docking results indicated that these compounds could bind to the amino acid residues in TYR catalytic pocket. These results proved that the proposed method is a feasible approach for screening of TYR inhibitors from plant extract.
KeywordAdsorbed hollow fibers Enzyme immobilization Inhibitor screening Pueraria lobata Tyrosinase
DOI10.1016/j.jpba.2020.113743
URLView the original
Language英语
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorZhao,Jing
Affiliation1.School of Chemistry and Chemical Engineering,Chongqing University,Chongqing,401331,China
2.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao SAR,China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhao,Cong Peng,Yin,Shi Jun,Chen,Guo Ying,et al. Adsorbed hollow fiber immobilized tyrosinase for the screening of enzyme inhibitors from Pueraria lobata extract[J]. Journal of Pharmaceutical and Biomedical Analysis,2021,193.
APA Zhao,Cong Peng.,Yin,Shi Jun.,Chen,Guo Ying.,Wang,Yuan.,Chen,Hua.,...&Yang,Feng Qing.(2021).Adsorbed hollow fiber immobilized tyrosinase for the screening of enzyme inhibitors from Pueraria lobata extract.Journal of Pharmaceutical and Biomedical Analysis,193.
MLA Zhao,Cong Peng,et al."Adsorbed hollow fiber immobilized tyrosinase for the screening of enzyme inhibitors from Pueraria lobata extract".Journal of Pharmaceutical and Biomedical Analysis 193(2021).
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