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Phanginin R induces cytoprotective autophagy via JNK/C-JUN signaling pathway in non-small cell lung cancer A549 cells
Zhang,Le Le1,2; Bao,Han2; Xu,Yu Lian2; Jiang,Xiao Ming2; Li,Wei1; Zou,Liang1; Lin,Li Gen2; Lu,Jin Jian2,3
2020
Source PublicationAnti-Cancer Agents in Medicinal Chemistry
ISSN1871-5206
Volume20Issue:8Pages:982-988
Abstract

Background: Cassane-type diterpenoids are widely distributed in the medical plants of genus Cae salpinia. To date, plenty of cassane diterpenoids have been isolated from the genus Caesalpinia, and some of them were documented to exhibit multiple biological activities. However, the effects of these compounds on autophagy have never been reported. Objective: To investigate the effects and mechanisms of the cassane diterpenoids including Phanginin R (PR) on autophagy in Non-Small Cell Lung Cancer (NSCLC) A549 cells. Methods: Western blot analysis and immunofluorescence assay were performed to investigate the effects of the compounds on autophagic flux in A549 cells. The pathway inhibitor and siRNA interference were used to investigate the mechanism of PR. MTT assay was performed to detect cell viability. Results: PR treatment upregulated the expression of phosphatidylethanolamine-modified microtubule-associated protein Light-Chain 3 (LC3-II) in A549 cells. Immunofluorescence assay showed that PR treatment increased the production of red-fluorescent puncta in mRFP-GFP-LC3 plasmid-transfected cells, indicating PR promoted autophagic flux in A549 cells. PR treatment activated the c-Jun N-terminal Kinase (JNK) signaling pathway while it did not affect the classical Akt/mammalian Target of Rapamycin (mTOR) pathway. Pretreatment with the JNK inhibitor SP600125 or siRNA targeting JNK or c-Jun suppressed PR-induced autophagy. In addition, cotreatment with the autophagy inhibitor Chloroquine (CQ) or inhibition of the JNK/c-Jun signaling pathway increased PR-induced cytotoxicity. Conclusion: PR induced cytoprotective autophagy in NSCLC A549 cells via the JNK/c-Jun signaling pathway, and autophagy inhibition could further improve the anti-cancer potential of PR.

KeywordAnti-cancer Autophagy C-jun Jnk Non-small Cell Lung Cancer Phanginin r
DOI10.2174/1871520620666200414095828
URLView the original
WOS Research AreaOncology ; Pharmacology & Pharmacy
WOS SubjectOncology ; Chemistry, Medicinal
WOS IDWOS:000552985200001
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.School of Medicine,Chengdu University,Chengdu,China
2.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao,China
3.College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhang,Le Le,Bao,Han,Xu,Yu Lian,et al. Phanginin R induces cytoprotective autophagy via JNK/C-JUN signaling pathway in non-small cell lung cancer A549 cells[J]. Anti-Cancer Agents in Medicinal Chemistry,2020,20(8):982-988.
APA Zhang,Le Le,Bao,Han,Xu,Yu Lian,Jiang,Xiao Ming,Li,Wei,Zou,Liang,Lin,Li Gen,&Lu,Jin Jian.(2020).Phanginin R induces cytoprotective autophagy via JNK/C-JUN signaling pathway in non-small cell lung cancer A549 cells.Anti-Cancer Agents in Medicinal Chemistry,20(8),982-988.
MLA Zhang,Le Le,et al."Phanginin R induces cytoprotective autophagy via JNK/C-JUN signaling pathway in non-small cell lung cancer A549 cells".Anti-Cancer Agents in Medicinal Chemistry 20.8(2020):982-988.
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