UM
SIRT3 acts as a positive autophagy regulator to promote lipid mobilization in adipocytes via activating AMPK
Zhang,Tian1; Liu,Jingxin1; Tong,Qiang2; Lin,Ligen1,3
2020-01-02
Source PublicationInternational Journal of Molecular Sciences
ISSN1661-6596
Volume21Issue:2
AbstractObesity is increasing at an alarming rate worldwide, which is characterized by the excessive accumulation of triglycerides in adipocytes. Emerging evidence has demonstrated that macroautophagy and chaperone-mediated autophagy (CMA) regulate lipid mobilization and play a key role in energy balance. Sirtuin 3 (SIRT3) is an NAD-dependent deacetylase, which is important in regulating macroautophagy and lipid metabolism. It is still unknown whether SIRT3 modulates macroautophagy and CMA in adipocytes. The current study found that macroautophagy was dynamically regulated during 3T3-L1 adipocyte differentiation, which coincided with SIRT3 expression. In mature adipocytes, overexpression of SIRT3 activated macroautophagy, mainly on lipid droplets (LDs), through activating the AMP-activated protein kinase (AMPK)-unc-51-like kinase 1 (ULK1) pathway, which in turn resulting in smaller LD size and reduced lipid accumulation. Moreover, SIRT3 overexpression induced the formation of perilipin-1 (PLN1)-heat shock cognate 71 kDa protein (HSC70)-lysosome-associated membrane protein 2 (LAMP2) complex, to activate CMA and cause the instability of LDs in adipocytes. In summary, we found SIRT3 is a positive regulator of macroautophagy and CMA in adipocytes, which might be a promising therapeutic target for treatment of obesity and its related metabolic dysfunction.
KeywordAMPK Chaperone-mediated autophagy Lipid metabolism Macroautophagy Sirtuin 3
DOI10.3390/ijms21020372
URLView the original
Language英语
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorLin,Ligen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Taipa,Avenida da Universidade,999078,Macao
2.Children’s Nutrition Research Center,Baylor College of Medicine,Houston,77030,United States
3.Zhuhai UM Science & Technology Research Institute,Zhuhai,519031,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhang,Tian,Liu,Jingxin,Tong,Qiang,et al. SIRT3 acts as a positive autophagy regulator to promote lipid mobilization in adipocytes via activating AMPK[J]. International Journal of Molecular Sciences,2020,21(2).
APA Zhang,Tian,Liu,Jingxin,Tong,Qiang,&Lin,Ligen.(2020).SIRT3 acts as a positive autophagy regulator to promote lipid mobilization in adipocytes via activating AMPK.International Journal of Molecular Sciences,21(2).
MLA Zhang,Tian,et al."SIRT3 acts as a positive autophagy regulator to promote lipid mobilization in adipocytes via activating AMPK".International Journal of Molecular Sciences 21.2(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang,Tian]'s Articles
[Liu,Jingxin]'s Articles
[Tong,Qiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang,Tian]'s Articles
[Liu,Jingxin]'s Articles
[Tong,Qiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang,Tian]'s Articles
[Liu,Jingxin]'s Articles
[Tong,Qiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.