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Quantitative characterization of ginsenoside biotransformation in panax notoginseng inflorescences and leaves by online two-dimensional liquid chromatography coupled to mass spectrometry
Ma,Li Juan1; Cao,Ji Liang1,2,3; Meng,Fan Cheng4; Wang,Sheng Peng1; Deng,Yun3; Wang,Yi Tao1; Li,Peng1; Wan,Jian Bo1
2020-04-22
Source PublicationJournal of Agricultural and Food Chemistry
ISSN0021-8561
Volume68Issue:19Pages:5327-5338
Abstract

Panax notoginseng inflorescences (PNI) and leaves (PNL) are commonly used as folk medicine and food supplements. In this study, an online two-dimensional hydrophilic interaction × reversed-phase liquid chromatography coupled to linear trap quadropole mass spectrometry method was developed to determine 24 ginsenosides, including two novel compounds, in PNI and PNL extracted by water and methanol. Our data demonstrated that ginsenosides Rd, Rc, Rb2, Rb3, Rb1, Ra2, Ra1, and Ra3 in both PNI and PNL extracted by water rather than methanol can be transformed to ginsenoside F2, notoginsenoside Fe, ginsenoside Rd2, notoginsenoside Fd, gypenoside XVII, PN02, PN01, and PN03, respectively, by selectively cleaving the β-(1→2)-glucosidic linkage at the C-3 position. Ginsenoside transformation was further verified to be mediated by the proteins isolated from samples. Additionally, the two newly discovered transformed products, namely, PN02 and PN03, were prepared and identified as novel compounds by nuclear magnetic resonance. Our findings provide new insight into the importance of extraction solvents on the component profile of natural products.

KeywordGinsenoside Transformation Online Hilic × Rp-ltq-ms Panax Notoginseng Inflorescences Panax Notoginseng Leaves
DOI10.1021/acs.jafc.0c01746
URLView the original
Indexed BySCIE
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000535254500006
Scopus ID2-s2.0-85084541861
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorLi,Peng; Wan,Jian Bo
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Taipa,999078,Macao
2.PU-UM Innovative Institute of Chinese Medical Sciences,Guangdong-Macau Traditional Chinese Medicine Technology Industrial Park Development Company,Limited,Hengqin New Area,Zhuhai, Guangdong,519031,China
3.College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China
4.College of Pharmaceutical Sciences,Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Ministry of Education),Southwest University,Chongqing,400715,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ma,Li Juan,Cao,Ji Liang,Meng,Fan Cheng,et al. Quantitative characterization of ginsenoside biotransformation in panax notoginseng inflorescences and leaves by online two-dimensional liquid chromatography coupled to mass spectrometry[J]. Journal of Agricultural and Food Chemistry,2020,68(19):5327-5338.
APA Ma,Li Juan,Cao,Ji Liang,Meng,Fan Cheng,Wang,Sheng Peng,Deng,Yun,Wang,Yi Tao,Li,Peng,&Wan,Jian Bo.(2020).Quantitative characterization of ginsenoside biotransformation in panax notoginseng inflorescences and leaves by online two-dimensional liquid chromatography coupled to mass spectrometry.Journal of Agricultural and Food Chemistry,68(19),5327-5338.
MLA Ma,Li Juan,et al."Quantitative characterization of ginsenoside biotransformation in panax notoginseng inflorescences and leaves by online two-dimensional liquid chromatography coupled to mass spectrometry".Journal of Agricultural and Food Chemistry 68.19(2020):5327-5338.
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